First study of pathogen load and localisation of ovine footrot using fluorescence in situ hybridisation (FISH)

Veterinary Microbiology
Luci A WitcombElizabeth M H Wellington

Abstract

Analysis of bacterial populations in situ provides insights into pathogen population dynamics and potential reservoirs for disease. Here we report a culture-independent study of ovine footrot (FR); a debilitating bacterial disease that has significant economic impact on sheep farming worldwide. Disease begins as an interdigital dermatitis (ID), which may then progress to separation of the hoof horn from the underlying epidermis causing severe footrot (SFR). Dichelobacter nodosus is the causative agent of ovine FR, however, the role of Fusobacterium necrophorum and other bacteria present in the environment and on the feet of sheep is less clear. The objective of this study was to use fluorescence in situ hybridisation (FISH) to detect, localise and quantify D. nodosus, F. necrophorum and the domain Bacteria from interdigital skin biopsies of healthy, ID- and SFR-affected feet. D. nodosus and F. necrophorum populations were restricted primarily to the epidermis, but both were detected more frequently in feet with ID or SFR than in healthy feet. D. nodosus cell counts were significantly higher in feet with ID and SFR (p<0.05) than healthy feet, whereas F. necrophorum cell counts were significantly higher only in feet with SFR (p<0...Continue Reading

References

Sep 19, 2000·Journal of Microbiological Methods·A Moter, U B Göbel
Nov 6, 2007·Current Opinion in Microbiology·Kevin D Young
Apr 17, 2008·Nature Reviews. Microbiology·Rudolf Amann, Bernhard M Fuchs
Jul 24, 2008·Environmental Microbiology·Alexander LoyMatthias Horn
Jun 27, 2009·Journal of Medical Microbiology·Geraint B RogersKenneth D Bruce
Mar 25, 2011·The ISME Journal·Leo A Calvo-BadoElizabeth M H Wellington
May 29, 2012·BMC Veterinary Research·Alessandro FoddaiJasmeet Kaler
Apr 5, 2014·Veterinary Microbiology·Maren Knappe-PoindeckerTerje Fjeldaas

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Citations

Sep 3, 2016·The Veterinary Record·Rachel Clifton, Laura Green
Jun 19, 2016·The Veterinary Record·G MaboniS Tötemeyer
Sep 30, 2017·Frontiers in Cellular and Infection Microbiology·Grazieli MaboniSabine Tötemeyer
Apr 13, 2021·The Veterinary Journal·Patrik ZanolariPeter Kuhnert

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Methods Mentioned

BETA
biopsies
PCR
biopsy

Software Mentioned

MLwiN
ImageJ
Leica LAS

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