First-trimester trophoblast cell model gene response to hypoxia

American Journal of Obstetrics and Gynecology
Nikki KoklanarisMen-Jean Lee

Abstract

Trophoblast invasion, which sets the stage for placentation and pregnancy outcome, likely occurs in a hypoxic environment. We used microarray technology in a trophoblast cell line to identify hypoxia-responsive genes that may impact placentation. An immortalized extravillous cytotrophoblast cell line, HTR-8/SVneo, was exposed to normoxia (20% oxygen) or hypoxia (1% oxygen) for 6 hours. Total RNA was harvested and prepared for microarray study. Quantitative reverse transcriptase polymerase chain reaction was performed for array confirmation. We confirmed the up- and down-regulation of 10 hypoxia-responsive genes using quantitative reverse transcriptase polymerase chain reaction. Ontologic gene categories that were found to be hypoxia-responsive included motility/migration, angiogenesis, and apoptosis. Specific genes that were found to be up-regulated in this first-trimester array (such as plasminogen activator inhibitor-1 and tissue inhibitor of metalloproteinase 3) have been described in preeclampsia. The hypoxia-responsive genes that we identified may be physiologic in early pregnancy. However, up-regulation of these same genes in later pregnancy augurs poorly.

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Citations

May 6, 2010·European Journal of Human Genetics : EJHG·Ryan Kc YuenWendy P Robinson
Nov 21, 2009·Human Reproduction Update·K G PringleC T Roberts
Nov 9, 2007·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Mara Lúcia Fonseca FerrazEumenia Costa da Cunha Castro
Nov 5, 2015·European Journal of Cell Biology·Amanda R HighetClaire T Roberts
Jan 24, 2007·American Journal of Obstetrics and Gynecology·Cathal McCarthyJoseph Vaughan
Sep 5, 2006·American Journal of Obstetrics and Gynecology·Stephen T ChasenRobin B Kalish
May 16, 2009·Endocrinology·Tomomi KotaniFumitaka Kikkawa
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Nariaki NakamuraYong Li
Apr 16, 2021·Oxidative Medicine and Cellular Longevity·Meihe LiHuimin Dang

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