Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway

Experimental and Therapeutic Medicine
Junjian Wang, Shaoxiang Huang

Abstract

Lung cancer is the most prevalent malignant tumor type in the developed world and the discovery of novel anti-tumor drugs is a research hotspot. Fisetin, a naturally occurring flavonoid, has been reported to have anti-cancer effects in multiple tumor types. The present study found that fisetin inhibited the growth and migration of non-small cell lung cancerin vitro. MTT, wound-healing, cell-matrix adhesion and Transwell assays were performed and demonstrated that fisetin suppressed proliferation, migration, adhesion and invasion, respectively. Flow cytometric analysis indicated that fisetin induced apoptosis in the A549 cell line by decreasing the expression of c-myc, cyclin-D1, cyclooxygenase-2, B cell lymphoma-2, CXC chemokine receptor type 4, cluster of differentiation 44 and metalloproteinase-2/9, increasing the expression of cyclin dependent kinase inhibitor (CDKN) 1A/B, CDKN2D and E-cadherin and increasing the activity of caspase-3/9 via targeting the extracellular signal-regulated kinase signaling pathway. The results provided comprehensive evidence for the anti-tumor effects of fisetin in non-small cell lung cancerin vitro, which may provide a novel approach for clinical treatment.

References

Nov 2, 1999·Biochemical and Biophysical Research Communications·Y Ishikawa, M Kitamura
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 4, 2005·Journal of Medicinal Chemistry·Heshu LuUrsula Schulze-Gahmen
Nov 30, 2005·Prostate Cancer and Prostatic Diseases·A Q HaddadL H Klotz
Oct 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pamela MaherKazuho Abe
Aug 12, 2009·International Journal of Cancer. Journal International Du Cancer·Imtiyaz MurtazaHasan Mukhtar
Sep 4, 2009·Journal of Agricultural and Food Chemistry·Yi-Chen LiaoTai-An Chiang
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Feb 3, 2015·Annals of Thoracic and Cardiovascular Surgery : Official Journal of the Association of Thoracic and Cardiovascular Surgeons of Asia·Keishi KawasakiYukihiro Yoshida
Jan 12, 2016·Therapeutic Advances in Medical Oncology·Ivana Sullivan, David Planchard
Jan 28, 2016·BMC Medical Informatics and Decision Making·Manuel LuqueCarlos Disdier

❮ Previous
Next ❯

Citations

Sep 5, 2019·International Journal of Molecular Sciences·Oana ZanoagaIoana Berindan-Neagoe
Oct 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mai KhaterHelen M I Osborn
Jun 5, 2021·Journal of Cell Communication and Signaling·Arun J Singh, Joe W Gray

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.