Fisetin Modulates Human Oral Squamous Cell Carcinoma Proliferation by Blocking PAK4 Signaling Pathways

Drug Design, Development and Therapy
Yanshu LiWei Dai

Abstract

Human oral squamous cell carcinoma (OSCC) is a major cause of mortality and morbidity worldwide. There is an urgent need to identify bioactive molecules and potential target genes that could inhibit carcinogenesis for OSCC therapy. Fisetin (3,7,3',4'-tetrahydroxyflavone), a naturally occurring flavonoid, has been previously shown to have anti-proliferative activities in OSCC; however, its molecular mechanism is unknown. Colony formation, cell viability, Boyden chamber, wound healing, and tumor xenograft assays were used to detect the impact of fisetin on OSCC cells in vitro and in vivo. Western blot analysis was used to examine the corresponding protein expression. Fisetin treatment significantly inhibited proliferation and promoted apoptosis by repressing PAK4 expression. Moreover, fisetin treatment attenuated cell migration by blocking PAK4 signaling pathways. In addition, the tumor xenograft showed anti-tumor growth effects of fisetin exposure in vivo. Fisetin may represent a potential therapeutic strategy for human OSCC by targeting PAK4 signaling pathways.

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Citations

Jul 25, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ammad Ahmad FarooqiBaojun Xu

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Methods Mentioned

BETA
electrophoresis
FACS
flow cytometry
xenograft

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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