Fisetin Suppresses Macrophage-Mediated Inflammatory Responses by Blockade of Src and Syk

Biomolecules & Therapeutics
Jun Ho KimJae Youl Cho

Abstract

Flavonoids, such as fisetin (3,7,3',4'-tetrahydroxyflavone), are plant secondary metabolites. It has been reported that fisetin is able to perform numerous pharmacological roles including anti-inflammatory, anti-microbial, and anti-cancer activities; however, the exact anti-inflammatory mechanism of fisetin is not understood. In this study, the pharmacological action modes of fisetin in lipopolysaccharide (LPS)-stimulated macrophage-like cells were elucidated by using immunoblotting analysis, kinase assays, and an overexpression strategy. Fisetin diminished the release of nitric oxide (NO) and reduced the mRNA levels of inducible NO synthase (iNOS), tumor necrosis factor (TNF)-α, and cyclooxygenase (COX)-2 in LPS-stimulated RAW264.7 cells without displaying cytotoxicity. This compound also blocked the nuclear translocation of p65/nuclear factor (NF)-κB. In agreement, the upstream phosphorylation events for NF-κB activation, composed of Src, Syk, and IκBα, were also reduced by fisetin. The phospho-Src level, triggered by overexpression of wild-type Src, was also inhibited by fisetin. Therefore, these results strongly suggest that fisetin can be considered a bioactive immunomodulatory compound with anti-inflammatory properties th...Continue Reading

References

Aug 1, 1988·Journal of Virological Methods·R PauwelsE De Clercq
Jan 1, 1996·Free Radical Biology & Medicine·C A Rice-EvansG Paganga
Feb 1, 2000·Baillière's Best Practice & Research. Clinical Rheumatology·W B van den Berg, B Bresnihan
Apr 5, 2003·Planta medica·Jeong-Min SeoByoung-Mog Kwon
Dec 24, 2011·Journal of Medicinal Chemistry·Chandramouli ChirutaPamela Maher
May 4, 2012·Archives of Pharmacal Research·Jin-Ah LeeHyeun Kyoo Shin
Nov 6, 2012·Antioxidants & Redox Signaling·Naghma KhanHasan Mukhtar
Jan 9, 2013·Journal of Ethnopharmacology·Deok JeongJae Youl Cho
Jun 12, 2013·Journal of Ethnopharmacology·Ju Young YoonJae Youl Cho
Nov 19, 2013·Biomolecules & Therapeutics·Cha Kyung YounYoung Jin Jeon
Feb 8, 2014·Journal of Ethnopharmacology·Deok JeongJae Youl Cho
Oct 17, 2014·The Journal of Pathology·Blanca Soler PalaciosAmaya Puig-Kröger
Nov 8, 2014·Journal of Ginseng Research·Mi-Yeon KimJae Youl Cho

❮ Previous
Next ❯

Citations

Jul 6, 2016·European Journal of Pharmacology·Thamaraiselvan Rengarajan, Nik Soriani Yaacob
Oct 18, 2016·Journal of Ginseng Research·Kwang-Soo BaekJae Youl Cho
Apr 4, 2017·The American Journal of Chinese Medicine·Jae Sik YuJae Youl Cho
Feb 22, 2017·Scientific Reports·Muhammad Jahangir HossenJae Youl Cho
Feb 22, 2018·The American Journal of Chinese Medicine·Woo Seok YangJae Youl Cho
Sep 6, 2018·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Jinfeng YangMyong Jo Kim
Apr 3, 2019·International Journal of Molecular Sciences·Eunji KimJae Youl Cho
May 9, 2019·Biomolecules·Dharambir KashyapSardul Singh Sandhu
Aug 24, 2017·The American Journal of Chinese Medicine·Woo Seok YangJae Youl Cho
Aug 29, 2020·Aging Cell·Brandon M HallAndrei V Gudkov
May 5, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Han Gyung KimJae Youl Cho
May 4, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Young-Su YiJae Youl Cho
Jul 15, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Sulgi YooJae Youl Cho
Jul 7, 2017·Mediators of Inflammation·Jae Gwang ParkJae Youl Cho
May 3, 2019·International Journal of Cancer. Journal International Du Cancer·Federica FrezzatoLivio Trentin
Jan 14, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Akash AhujaJae Youl Cho
Aug 16, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·You Jin KimJae Youl Cho
Feb 20, 2021·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Jozaa Z ALTamimiAmmar M Al-Farga
May 11, 2021·Journal of Biomolecular Structure & Dynamics·Navid JamaliJavad Saffari-Chaleshtori
Jun 9, 2021·Critical Reviews in Food Science and Nutrition·Hanne VissenaekensJohn Van Camp
Jun 27, 2021·Journal of Cardiovascular Pharmacology·Johnna F VargheseUmesh C S Yadav

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear translocation
transfection
profiler

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.