PMID: 11331672May 2, 2001Paper

FISH analysis of the chromosomal status of spermatozoa from three men with 45,XY,der(13;14)(q10;q10) karyotype

Molecular Human Reproduction
F MorelJ L Bresson

Abstract

Meiotic segregation of chromosomes 13 and 14 was studied in the ejaculated spermatozoa of three men carrying a translocation der(13;14)(q10;q10). The spermatozoa of these patients and of a donor with a normal 46,XY karyotype (control) were analysed by two-colour fluorescent in-situ hybridization (FISH) with specific chromosomal painting of chromosomes 13 and 14, by two-colour FISH detecting chromosomes 18 and 21 and by triple-colour FISH for chromosomes X, Y and 8. For patients 1, 2 and 3, respectively, 81.34, 82.60 and 88.90% of the analysed nuclei showed normal or balanced chromosomal status, resulting from the alternate segregation of the translocation. The rates of spermatozoa with an unbalanced status (disomy and nullisomy, 13 or 14) resulting from the adjacent mode of segregation were estimated respectively at 18.06, 16.32 and 10.80 (for patients 1, 2 and 3). Additional colour FISH analysis with probes specific for chromosomes X, Y, 8, 18 and 21 showed a significant increase in some disomy frequencies (8, 18, 21, X and Y for patient 1, only 18 for patient 2) in comparison with the control. These results would seem to indicate an interchromosomal effect.

References

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Aug 13, 1999·Archives of Andrology·F MorelJ L Bresson

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Citations

Mar 31, 2011·Journal of Assisted Reproduction and Genetics·Manel MahjoubAli Saad
Jul 31, 2013·Journal of Assisted Reproduction and Genetics·Larysa Y PylypNadiya M Bilko
Mar 9, 2011·Journal of Applied Genetics·Chao FuRui-Zhi Liu
Jun 18, 2010·Journal of Human Genetics·Ester AntonFrancesca Vidal
Jun 7, 2008·Human Reproduction Update·R H Martin
Dec 3, 2009·Molecular Cytogenetics·Maria S Juchniuk de VozziLucia Martelli
Jun 6, 2006·Asian Journal of Andrology·Ozge OzalpFeride Iffet Sahin
Aug 20, 2005·Asian Journal of Andrology·Zaida SarrateFrancesca Vidal
May 19, 2007·Reproductive Biomedicine Online·Gabor HuszarSinan Ozkavukcu
Jul 24, 2004·International Journal of Andrology·Frédéric MorelMarc De Braekeleer
Nov 23, 2005·International Journal of Andrology·N Douet-GuilbertF Morel
Nov 19, 2011·Systems Biology in Reproductive Medicine·Ester AntonJoan Blanco
May 15, 2007·Current Opinion in Obstetrics & Gynecology·Elizabeth E Puscheck, Rajasingam S Jeyendran
Sep 30, 2005·Cytogenetic and Genome Research·C RouxJ L Bresson
Sep 30, 2005·Cytogenetic and Genome Research·E AntonF Vidal
Mar 10, 2006·Molecular Human Reproduction·Gonul OgurInge Liebaers
Jun 7, 2014·Annals of Pediatric Endocrinology & Metabolism·Byung Ho ChoiCheol Woo Ko
Jun 10, 2020·Cellular and Molecular Life Sciences : CMLS·Ewa WilandMaciej Kurpisz
Oct 20, 2020·Reproductive Medicine and Biology·Shinnosuke KurodaYasushi Yumura

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