FISH and tips: a large scale analysis of automated versus manual scoring for sperm aneuploidy detection

Basic and Clinical Andrology
Guillaume MartinezSylviane Hennebicq

Abstract

Approximately 1% of the spermatozoa found in ejaculate of healthy men are aneuploid and this rate increases in the population of subfertile and infertile men. Moreover, fertilization with these aneuploid sperm can lead to impaired embryo development. Fluorescent In Situ Hybridization (FISH) is the common cytogenetic tool used for aneuploidy screening on sperm. However, it is a time-consuming technique and cytogenetic or in vitro fertilization laboratories cannot routinely use it and face the increasing demand of such analyses before Assisted Reproductive Techniques (ART). As automation can be a clue for routine practice, this study compares manual and automated scoring of sperm aneuploidy rates using a Metafer Metasystems® device. The results obtained also contribute to global data about FISH on sperm cells. We recruited 100 men addressed for sperm cryopreservation. They all signed an informed consent to participate in the study. 29 men were donors or consulted before vasectomy (control group) and 71 were suffering of Hodgkin's disease or non Hodgkin lymphoma (patient group). One semen sample was collected for each patient, analyzed according to WHO criteria and prepared for a triple-color FISH using centromeric probes for chro...Continue Reading

References

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Citations

Jul 6, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Francesca BranchMelissa J Perry
Jan 4, 2019·Journal of Assisted Reproduction and Genetics·Elena García-MengualXavier Vendrell
Oct 13, 2016·Environmental Health : a Global Access Science Source·Daniele MandrioliMelissa J Perry
Nov 20, 2018·Systems Biology in Reproductive Medicine·Ying MaXiaodong Xie

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

Metafer
GAMATOX
Spot AX system
BioImage and
Atto Imaging Vision

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