FISH-Based Markers Enable Identification of Chromosomes Derived From Tetraploid Thinopyrum elongatum in Hybrid Lines

Frontiers in Plant Science
Daiyan LiHouyang Kang

Abstract

Tetraploid Thinopyrum elongatum, which has superior abiotic stress tolerance characteristics, and exhibits resistance to stripe rust, powdery mildew, and Fusarium head blight, is a wild relative of wheat and a promising source of novel genes for wheat improvement. Currently, a high-resolution Fluorescence in situ hybridization (FISH) karyotype of tetraploid Th. elongatum is not available. To develop chromosome-specific FISH-based markers, the hexaploid Trititrigia 8801 and two accessions of tetraploid Th. elongatum were characterized by different repetitive sequences probes. We found that all E-genome chromosomes could be unambiguously identified using a combination of pSc119.2, pTa535, pTa71, and pTa713 repeats, and the E-genome chromosomes of the wild accessions and the partial amphiploid failed to exhibit any significant variation in the probe hybridization patterns. To verify the validation of these markers, the chromosome constitution of eight wheat- Th. elongatum hybrid derivatives were analyzed. We revealed that these probes could quickly detect wheat and tetraploid Th. elongatum chromosomes in hybrid lines. K16-712-1-2 was a 1E (1D) chromosome substitution line, K16-681-4 was a 2E disomic chromosome addition line, K16-5...Continue Reading

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Citations

Dec 18, 2019·Comparative Cytogenetics·Ekaterina D BadaevaAlexander V Agafonov
Dec 20, 2018·Frontiers in Plant Science·Alevtina S Ruban, Ekaterina D Badaeva
Apr 28, 2019·International Journal of Molecular Sciences·Wei XiShulan Fu
Feb 18, 2021·Genome Génome / Conseil National De Recherches Canada·Yi DaiJianmin Chen
Apr 15, 2021·Genome Génome / Conseil National De Recherches Canada·Xiu YangHouyang Kang

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Methods Mentioned

BETA
PCR
electrophoresis
eight hybrid

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