Fish eco-genotoxicology: Comet and micronucleus assay in fish erythrocytes as in situ biomarker of freshwater pollution

Saudi Journal of Biological Sciences
Bilal HussainShahid Mahboob

Abstract

Owing to white meat productionLabeo rohitahave vast economic importance, but its population has been reduced drastically in River Chenab due to pollution. Atomic absorption spectrophotometry showed a merciless toxicity level of Cd, Cu, Mn, Zn, Pb, Cr, Sn and Hg. Comet assay results indicated significant (p < .05) DNA fragmentation inLabeo rohitaas 42.21 ± 2.06%, 31.26 ± 2.41% and 21.84 ± 2.21% DNA in comet tail, tail moment as 17.71 ± 1.79, 10.30 ± 1.78 and 7.81 ± 1.56, olive moment as 13.58 ± 1.306, 8.10 ± 1.04 and 5.88 ± 0.06, respectively, from three different polluted sites on the river. Micronucleus assay showed similar findings of single micronucleus induction (MN) as 50.00 ± 6.30‰, double MN 14.40 ± 2.56‰, while nuclear abnormalities (NA) were found as 150.00 ± 2.92‰. These higher frequencies of MN induction and NA were found to be the cause of reduction of 96% of the population of this fish species in an experimental area of the River Chenab. This fish species has been found near extinction through the length of the river Chenab and few specimens in rainy seasons if restored by flood, may die in sugarcane mill season. Due to sweeping extinctionLabeo rohitashowed the highest sensitivity for pollution and could be used as...Continue Reading

Citations

May 2, 2020·Bulletin of Environmental Contamination and Toxicology·Seda Kontaş, Derya Bostancı
Jun 4, 2020·Environmental Science and Pollution Research International·Yichen QiuDenghang Yu
Apr 26, 2019·Environmental Science and Pollution Research International·Marie-Laure AcolasJérôme Cachot
Jul 6, 2020·The Science of the Total Environment·Amanda Pereira da Costa AraújoGuilherme Malafaia
Jul 29, 2021·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Atsler Luana LehunAna Carolina de Deus Bueno Krawczyk

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Methods Mentioned

BETA
atomic absorption spectrophotometry
electrophoresis

Software Mentioned

SPSS
TriTek Comet
TriTek Comet [UNK] Freeware

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