FISH-mapping of rDNAs and Arabidopsis BACs on pachytene complements of selected Brassicas

Genome Génome / Conseil National De Recherches Canada
Piotr A Ziolkowski, J Sadowski

Abstract

To improve resolution of physical mapping on Brassica chromosomes, we have chosen the pachytene stage of meiosis where incompletely condensed bivalents are much longer than their counterparts at mitotic metaphase. Mapping with 5S and 45S rDNA sequences demonstrated the advantage of pachytene chromosomes in efficient physical mapping and confirmed the presence of a novel 5S rDNA locus in Brassica oleracea, initially identified by genetic mapping using restriction fragment length polymorphism (RFLP). Fluorescence in situ hybridization (FISH) analysis visualized the presence of the third 5S rDNA locus on the long arm of chromosome C2 and confirmed the earlier reports of two 45S rDNA loci in the B. oleracea genome. FISH mapping of low-copy sequences from the Arabidopsis thaliana bacterial artificial chromosome (BAC) clones on the B. oleracea chromosomes confirmed the expectation of efficient and precise physical mapping of meiotic bivalents based on data available from A. thaliana and indicated conserved organization of these two BAC sequences on two B. oleracea chromosomes. Based on the heterologous in situ hybridization with BACs and their mapping applied to long pachytene bivalents, a new approach in comparative analysis of Bras...Continue Reading

References

Jan 14, 1998·Plant Molecular Biology·M L ChyeJ Xu
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·P FranszG Jones
Jul 17, 1999·Trends in Plant Science· Hans de Jong JP Zabel
Jul 19, 2000·The Plant Cell·G BlancM Delseny
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Jan 1, 1960·Chromosoma·G ROEBBELEN
Aug 1, 1997·Genome Génome / Conseil National De Recherches Canada·R J SnowdonA Köhler
Aug 1, 1993·Genome Génome / Conseil National De Recherches Canada·J Maluszynska, J S Heslop-Harrison
Aug 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S F Kianian, C F Quiros
Mar 1, 1998·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K FukuiM Yamabe
Mar 1, 1998·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J Sadowski, C F Quiros

❮ Previous
Next ❯

Citations

Sep 15, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Jaroslav JandaJaroslav Dolezel
Sep 15, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Dal-Hoe KooJae-Wook Bang
Mar 10, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Robert HasterokWaheeb K Heneen
Feb 8, 2005·Journal of Molecular Evolution·Anton Nekrutenko
Feb 14, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Rod J Snowdon
Nov 19, 2013·AoB Plants·Ana Amélia Sanchez Iacia, Cecília A F Pinto-Maglio
Mar 23, 2005·Genome Research·Martin A LysakIngo Schubert
Apr 20, 2005·Genome Génome / Conseil National De Recherches Canada·Hoda B M AliIngo Schubert
Nov 18, 2006·Genome Génome / Conseil National De Recherches Canada·Jiming Jiang, Bikram S Gill
Apr 17, 2009·Genome Génome / Conseil National De Recherches Canada·Jiuhuan FengSteven S Xu
Mar 9, 2005·Cytogenetic and Genome Research·J Maluszynska, R Hasterok
May 28, 2008·Cytogenetic and Genome Research·T RibeiroL Morais-Cecíli
Jul 11, 2006·The Plant Journal : for Cell and Molecular Biology·Piotr A ZiolkowskiJan Sadowski
Mar 24, 2006·Yi chuan xue bao = Acta genetica Sinica·Kun YangXiao-Jia Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.