PMID: 9172071Jun 1, 1997Paper

Fish muscle cytoskeleton integrity is not dependent on intact thin filaments

Journal of Muscle Research and Cell Motility
R G TaylorA Ouali

Abstract

Striated muscle cytoskeleton was studied by ultrastructure and electrophoresis. Treatment of sea bass white muscle myofibrils and glycerinated fibres with calpain caused disruption of costameres, intermediate filaments, and Z-line, without altering sarcomeres. V8 protease also caused loss of costameres and Z-line, and disrupted sarcomeres without affecting the intermediate filaments. Recombinant lipase caused loss of Z-lines and also sarcolemma detachment, without changing sarcomeres or intermediate filaments. DNase-1 removed thin filaments and partially removed Z-lines while leaving intact the sarcolemma attachments and intermediate filaments. Calpain, V8 protease, lipase and DNase-1 treatments induced extensive loss of alpha-actinin from the Z-line, which could be related to titin cleavage (calpain, V8), phosphoinositide hydrolysis (lipase), and actin depolymerisation (DNase-1). These results show that the cytoskeletal components are independent of intact thin filaments.

Citations

Aug 4, 2006·The FEBS Journal·Marie-Christine Lebart, Yves Benyamin
Jan 19, 1999·Annual Review of Cell and Developmental Biology·R Littlefield, V M Fowler
Jul 7, 2015·Critical Reviews in Food Science and Nutrition·Arashdeep SinghSavita Sharma
Apr 21, 2016·PloS One·Alicia A CassidySimon G Lamarre
Dec 10, 2002·Annual Review of Physiology·Helen L Yin, Paul A Janmey
May 1, 2000·Journal of the Science of Food and Agriculture·E Heather MorrisonPeter P Purslow
Mar 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Avtar Singh, Soottawat Benjakul
Nov 17, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Thomas P Mommsen
Apr 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·O KwiateckC Roustan
Dec 18, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Peter P Purslow

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