PMID: 9188647Jul 1, 1997Paper

Fish nodavirus lytic cycle and semipermissive expression in mammalian and fish cell cultures

Journal of Virology
C DelsertM Comps

Abstract

In this study, Dicentrarchus labrax encephalitis virus (DlEV), which causes sea bass encephalitis, was propagated in cell culture, thus allowing study of its lytic cycle. DlEV infection of mammalian and fish cells induced different patterns of expression of capsid proteins, which were assembled as virus-like particles, accumulating in the cytoplasm either as diffuse masses or in vesicles, as shown by electron microscopy. These particles correspond to virions, as shown by their ability to induce secondary infection. Fish cells proved to be more permissive for DlEV than mammalian cells, although virus yield remained low. RNA analysis of infected sea bass cells revealed DlEV RNA3, in addition to genomic RNA1 and RNA2, and the presence of the RNA2 minus strand, thus demonstrating the replication of the DlEV genome. In addition, DlEV RNA-dependent RNA polymerase was associated with mature virions even after purification by a CsCl gradient, but it was dissociated when capsids were destabilized. In addition to providing more information about the relatedness of DlEV to the members of the family Nodaviridae, this study shows that fish nodaviruses may not be able to infect as wide a variety of cells as insect nodaviruses can.

References

Oct 1, 1976·The Journal of General Virology·J F Longworth, G P Carey
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Citations

Feb 13, 2009·Journal of Virology·Joseph G VictoriaEric Delwart
Sep 12, 2001·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·S Essbauer, W Ahne
Jul 18, 2001·The Journal of General Virology·K N JohnsonL A Ball
Aug 20, 2002·The Journal of General Virology·John V Krondiris, Diamantis C Sideris
Feb 9, 2013·Archives of Virology·V Sarath BabuA S Sahul Hameed
Dec 2, 1999·The Journal of General Virology·T Nagai, T Nishizawa
May 15, 2018·Transboundary and Emerging Diseases·Y C KimH D Jeong
Sep 7, 2018·Veterinary Research·Sandra SoutoIsabel Bandín
Jan 30, 2019·Journal of Fish Diseases·Young Chul KimHyun Do Jeong
Oct 27, 1999·Journal of Invertebrate Pathology·J L ZeddamA Lagnaoui

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