Fish oil alleviates liver injury induced by intestinal ischemia/reperfusion via AMPK/SIRT-1/autophagy pathway

World Journal of Gastroenterology : WJG
Hui-Rong JingYun Zhou

Abstract

To evaluate whether fish oil (FO) can protect liver injury induced by intestinal ischemia/reperfusion (I/R) via the AMPK/SIRT-1/autophagy pathway. Ischemia in Wistar rats was induced by superior mesenteric artery occlusion for 60 min and reperfusion for 240 min. One milliliter per day of FO emulsion or normal saline was administered by intraperitoneal injection for 5 consecutive days to each animal. Animals were sacrificed at the end of reperfusion. Blood and tissue samples were collected for analyses. AMPK, SIRT-1, and Beclin-1 expression was determined in lipopolysaccharide (LPS)-stimulated HepG2 cells with or without FO emulsion treatment. Intestinal I/R induced significant liver morphological changes and increased serum alanine aminotransferase and aspartate aminotransferase levels. Expression of p-AMPK/AMPK, SIRT-1, and autophagy markers was decreased whereas tumor necrosis factor-α (TNF-α) and malonaldehyde (MDA) were increased. FO emulsion blocked the changes of the above indicators effectively. Besides, in LPS-stimulated HepG2 cells, small interfering RNA (siRNA) targeting AMPK impaired the FO induced increase of p-AMPK, SIRT-1, and Beclin-1 and decrease of TNF-α and MDA. SIRT-1 siRNA impaired the increase of SIRT-1 and...Continue Reading

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Citations

Jun 14, 2019·Oxidative Medicine and Cellular Longevity·Xin FanJun Zhou
Dec 12, 2019·Environmental Science and Pollution Research International·Manar G Helal, Dalia H El-Kashef
Aug 16, 2019·Bioscience Reports·Jia LiuJiyan Leng
Dec 31, 2019·The Journal of Nutritional Biochemistry·Nien-Shan LuSuh-Ching Yang
Mar 20, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun WangGuosheng Wu
Apr 27, 2021·Frontiers in Cell and Developmental Biology·QingJian LiangWeiNa Wang
Nov 5, 2021·Journal of Oleo Science·Suh-Ching Yang
Jan 6, 2022·Journal of Cellular and Molecular Medicine·Xiaoning YangFanghua Gong

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Methods Mentioned

BETA
light microscopy
enzyme-linked immunosorbent assay
MDA
ELISA
transfection
gene knockout

Software Mentioned

Gel
- Pro Analyzer

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