Fish Oil Derived Omega 3 Fatty Acids Suppress Adipose NLRP3 Inflammasome Signaling in Human Obesity

Journal of the Endocrine Society
Kailey Roberts LeeRachana Shah

Abstract

The NRLP3 inflammasome is a multiprotein danger-sensing complex that serves as a critical link between obesity-related adipose inflammation and insulin resistance and has been shown in animal models to be inhibited by fish oil-derived long chain omega-3 polyunsaturated fatty acids (n-3 PUFA). We conducted a clinical trial and in vitro experiments to test our hypothesis that n-3 PUFA suppress NLRP3 inflammasome in human obesity through downregulation of inflammasome gene expression in adipocytes and macrophages. Placebo-controlled clinical trial and in vitro coculture experiments with primary human adipocytes (from biopsy specimens) and human THP-1 monocyte-derived macrophages treated with eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) vs vehicle control. General community, research laboratory. Obese (body mass index ≥ 30 kg/m2), nondiabetic males and females age 18 to 50. N = 25. Clinical trial: Eight-week treatment with 4 g Lovaza (EPA and DHA) or placebo. Cells culture: EPA and/or DHA at 100 µg/mL or vehicle control in culture medium. Adipose tissue or adipocyte/macrophage mRNA expression of IL-1β and IL-18 and circulating IL-18 levels. Treatment of obese human subjects with fish oil supplements reduced express...Continue Reading

References

Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·E Stylianou, J Saklatvala
Dec 13, 2005·The Journal of Clinical Investigation·Stuart P WeisbergAnthony W Ferrante
Jan 4, 2007·The Journal of Clinical Investigation·Carey N LumengAlan R Saltiel
Jun 15, 2007·Nature·Jan M SchwabCharles N Serhan
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Sep 23, 2008·European Journal of Nutrition·Nerea Pérez-EcharriMaría Jesus Moreno-Aliaga
Jul 8, 2009·Diabetes·Rachana ShahMuredach P Reilly
Jul 28, 2009·Nature Medicine·Shawn WinerH-Michael Dosch
Aug 1, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Carine DuffautAnne Bouloumié
Aug 8, 2009·Nature Medicine·Carey N LumengAlan R Saltiel
Oct 16, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vassilia-Ismini AlexakiElias Castanas
Oct 20, 2009·The Journal of Clinical Endocrinology and Metabolism·Judith Aron-WisnewskyKarine Clément
Dec 10, 2009·The Journal of Experimental Medicine·Daniel J WestcottCarey N Lumeng
Dec 15, 2010·The Journal of Nutritional Biochemistry·Michael J PuglisiViswanathan Saraswathi
Jan 11, 2011·Nature Medicine·Bolormaa VandanmagsarVishwa Deep Dixit
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rinke StienstraThirumala-Devi Kanneganti
May 25, 2013·Nature Reviews. Immunology·Eicke LatzAndrea Stutz
Sep 2, 2014·Biochemical Pharmacology·Sylvie Legrand-PoelsJacques Piette
Apr 17, 2015·Immunological Reviews·Si Ming Man, Thirumala-Devi Kanneganti
Jun 30, 2015·Nature Medicine·Haitao GuoJenny P-Y Ting
Aug 11, 2016·F1000Research·Tomasz PróchnickiEicke Latz
Aug 3, 2017·Metabolism: Clinical and Experimental·Jakeline RheinheimerDaisy Crispim
Aug 23, 2017·Annual Review of Nutrition·Jessica C RalstonHelen M Roche

❮ Previous
Next ❯

Citations

Jul 18, 2019·Current Opinion in Clinical Nutrition and Metabolic Care·James MorganSimon Lal
Mar 7, 2020·The Proceedings of the Nutrition Society·Jacques Delarue
Apr 10, 2020·The Journal of Nutrition·Kate J Claycombe-LarsonJames N Roemmich
Jun 17, 2020·Annual Review of Nutrition·Nishan Sudheera KalupahanaNaima Moustaid-Moussa
Oct 3, 2020·Molecular Neurobiology·Gabriela Cruz-Carrillo, Alberto Camacho-Morales
Dec 11, 2019·Nutrients·Urszula RadzikowskaMilena Sokolowska
Apr 18, 2021·The International Journal of Biochemistry & Cell Biology·Salih KucukClaudio Mauro
Aug 28, 2021·Frontiers in Nutrition·Barry Sears, Asish K Saha

❮ Previous
Next ❯

Methods Mentioned

BETA
pregnancy test
blood draw
blood
biopsy
enzyme-linked immunosorbent assay
PMA
PCR
transgenic

Clinical Trials Mentioned

NCT02010359

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here