Fish testicular 11beta-hydroxylase: cDNA cloning and mRNA expression during spermatogenesis

FEBS Letters
J Q JiangYoshitaka Nagahama

Abstract

We isolated and characterized a cDNA encoding testicular 11beta-hydroxylase, cytochrome P450(11beta) from the Japanese eel (Anguilla japonica) testis. The cDNA contains an open reading frame that encodes a protein of 511 amino acids. The predicted amino acid sequence shares 38-48% homology with those of adrenal P450(11beta) from mammals and frog. Transient expression in COS 1 cells confirmed that the protein encoded by this cDNA had P450(11beta) activity. Northern blotting revealed a single 1.8 kb long transcript of P450(11beta). This transcript was not found in immature eel testes prior to an injection with human chorionic gonadotropin (hCG), but it was present in eel testes after hCG injection.

References

Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T MiuraY Nagahama
May 31, 1990·Biochemical and Biophysical Research Communications·N MatsukawaM Okamoto
Mar 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·T SunM Okamoto
Feb 1, 1995·Biology of Reproduction·A H Payne, G L Youngblood
Jan 2, 1995·Biochimica Et Biophysica Acta·W C BoonJ P Coghlan

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Citations

Jan 19, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Martín BlascoDenise Vizziano-Cantonnet
Dec 24, 2014·General and Comparative Endocrinology·Adimoolam ArunaChing-Fong Chang
Mar 30, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·X G Wang, L Orban
Apr 3, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·A J ConleyA L Hughes
Jan 16, 2020·Fish Physiology and Biochemistry·Anbazhagan Rajakumar, Balasubramanian Senthilkumaran
Apr 9, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Juilee RegeWilliam E Rainey
Mar 19, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Yukinori KazetoShinji Adachi

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