Fish TRIM39 regulates cell cycle progression and exerts its antiviral function against iridovirus and nodavirus

Fish & Shellfish Immunology
Wei WangXiaohong Huang

Abstract

The tripartite motif (TRIM)-containing proteins exert important immune regulatory roles through regulating different signaling pathways in response to different stimuli. TRIM39, a member of the TRIM family, is a RING domain-containing E3 ubiquitin ligase which could regulate cell cycle progression and apoptosis. However, the antiviral activity of TRIM39 is not explored. Here, a TRIM39 homolog from grouper, Epinephelus coioides (EcTRIM39) was cloned, and its effects on cell cycle progression and fish virus replication were investigated. The full-length EcTRIM39 cDNA was composed of 2535 bp and encoded a polypeptide of 543 amino acids with 70% identity with TRIM39 homologs from bicolor damselfish. Amino acid alignment analysis indicated that EcTRIM39 contained a RING finger, B-box and SPRY domain. Expression profile analysis revealed that EcTRIM39 was abundant in intestine, spleen and skin. Upon different stimuli in vivo, the EcTRIM39 transcript was obviously up-regulated after challenging with Singapore grouper iridovirus (SGIV), and polyinosinic-polycytidylic acid (poly I:C). Using fluorescence microscopy, we found that EcTRIM39 localized in the cytoplasm and formed aggregates in grouper spleen (GS) cells. The ectopic expressio...Continue Reading

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Citations

May 29, 2016·Fish & Shellfish Immunology·Youhua HuangQiwei Qin
Jun 9, 2016·Fish & Shellfish Immunology·Ying YangXiaohong Huang
Jul 21, 2016·Fish & Shellfish Immunology·Yepin YuQiwei Qin
Jul 28, 2016·Developmental and Comparative Immunology·Sarah J Poynter, Stephanie J DeWitte-Orr
Aug 20, 2016·Fish & Shellfish Immunology·Ying YangXiaohong Huang
Oct 30, 2019·The Journal of General Virology·Adam Hage, Ricardo Rajsbaum
Jun 13, 2017·Frontiers in Immunology·Christelle LangevinPierre Boudinot
Oct 4, 2017·PeerJ·Chean Yeah YongWen Siang Tan

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