DOI: 10.1101/494971Dec 17, 2018Paper

FishNET: An automated relational database for zebrafish colony management.

BioRxiv : the Preprint Server for Biology
Abiud Cantu GutierrezJoshua D Wythe

Abstract

The zebrafish Danio rerio is a powerful model system to study the genetics of development and disease. However, maintenance of zebrafish husbandry records is both time intensive and laborious, and a standardized way to manage and track the large amount of unique lines in a given laboratory or centralized facility has not been embraced by the field. Here we present FishNet, an intuitive, open source, relational database for managing data and information related to zebrafish husbandry and maintenance. By creating a virtual facility, FishNET enables users to remotely inspect the rooms, racks, tanks and lines within a given facility. Importantly, FishNET scales from one laboratory, to an entire facility with several laboratories, to multiple facilities, generating a cohesive laboratory and community-based platform. Automated data entry eliminates confusion regarding line nomenclature and streamlines maintenance of individual lines, while flexible query forms allow researchers to retrieve database records based on user-defined criteria. FishNet also links associated embryonic and adult biological samples with data, such as genotyping results or confocal images, to enable robust and efficient colony management and storage of laborato...Continue Reading

Related Concepts

Confusion
Embryo
Laboratory
Research
Research Personnel
Zebrafish
Cell Line, Tumor
Microscopy, Confocal
Disease Management
Genotype Determination

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