Fission yeast Cyk3p is a transglutaminase-like protein that participates in cytokinesis and cell morphogenesis.

Molecular Biology of the Cell
Luther W PollardMatthew Lord

Abstract

Cell morphogenesis is a complex process that relies on a diverse array of proteins and pathways. We have identified a transglutaminase-like protein (Cyk3p) that functions in fission yeast morphogenesis. The phenotype of a cyk3 knockout strain indicates a primary role for Cyk3p in cytokinesis. Correspondingly, Cyk3p localizes both to the actomyosin contractile ring and the division septum, promoting ring constriction, septation, and subsequent cell separation following ring disassembly. In addition, Cyk3p localizes to polarized growth sites and plays a role in cell shape determination, and it also appears to contribute to cell integrity during stationary phase, given its accumulation as dynamic puncta at the cortex of such cells. Our results and the conservation of Cyk3p across fungi point to a role in cell wall synthesis and remodeling. Cyk3p possesses a transglutaminase domain that is essential for function, even though it lacks the catalytic active site. In a wider sense, our work illustrates the physiological importance of inactive members of the transglutaminase family, which are found throughout eukaryotes. We suggest that the proposed evolution of animal transglutaminase cross-linking activity from ancestral bacterial thi...Continue Reading

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Citations

Jul 24, 2013·The Journal of Cell Biology·Masayuki OnishiJohn R Pringle
Oct 25, 2012·PLoS Genetics·K Adam Bohnert, Kathleen L Gould
Feb 26, 2015·Cytoskeleton·Joseph E ClaytonMatthew Lord
Sep 2, 2014·Current Biology : CB·Evelyn Yaqiong TaoMohan K Balasubramanian
Sep 16, 2017·ELife·Nathan A McDonaldKathleen L Gould
Feb 27, 2020·Molecular Biology of the Cell·Rahul BhattacharjeeKathleen L Gould
Oct 11, 2019·The Journal of Cell Biology·Mariona RamosJuan Carlos Ribas
Jul 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Masayuki OnishiJohn R Pringle
Sep 8, 2012·Journal of Cell Science·Asli DevrekanliKarim Labib

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