Fission yeast dim1(+) encodes a functionally conserved polypeptide essential for mitosis

The Journal of Cell Biology
L D Berry, Kathleen L Gould

Abstract

In a screen for second site mutations capable of reducing the restrictive temperature of the fission yeast mutant cdc2-D217N, we have isolated a novel temperature-sensitive mutant, dim1-35. When shifted to restrictive temperature, dim1-35 mutant cells arrest before entry into mitosis or proceed through mitosis in the absence of nuclear division, demonstrating an uncoupling of proper DNA segregation from other cell cycle events. Deletion of dim1 from the Schizosaccharomyces pombe genome produces a lethal G2 arrest phenotype. Lethality is rescued by overexpression of the mouse dim1 homolog, mdim1. Likewise, deletion of the Saccharomyces cerevisiae dim1 homolog, CDH1, is lethal. Both mdim1 and dim1(+) are capable of rescuing lethality in the cdh1::HIS3 mutant. Although dim1-35 displays no striking genetic interactions with various other G2/M or mitotic mutants, dim1-35 cells incubated at restrictive temperature arrest with low histone H1 kinase activity. Morevoer, dim1-35 displays sensitivity to the microtubule destabilizing drug, thiabendazole (TBZ). We conclude that Dim1p plays a fundamental, evolutionarily conserved role as a protein essential for entry into mitosis as well as for chromosome segregation during mitosis. Based on...Continue Reading

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Citations

Jan 25, 2002·Journal of Cellular Physiology·Katya RavidMatthew R Jones
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Dec 8, 2019·Scientific Reports·Henry Akrofi DokuDong-Sun Lee
May 27, 2004·The Journal of Biological Chemistry·Xiaojing SunYongfeng Shang

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Datasets Mentioned

BETA
W85636

Methods Mentioned

BETA
PCR
flow cytometry
fluoresence microscopy
restriction digest
spore
immunoprecipitation
light microscopy

Software Mentioned

ImageQuant
MD

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