Fission yeast mutants affecting telomere clustering and meiosis-specific spindle pole body integrity.

Genetics
Ye JinW Zacheus Cande

Abstract

In meiotic prophase of many eukaryotic organisms, telomeres attach to the nuclear envelope and form a polarized configuration called the bouquet. Bouquet formation is hypothesized to facilitate homologous chromosome pairing. In fission yeast, bouquet formation and telomere clustering occurs in karyogamy and persists throughout the horsetail stage. Here we report the isolation and characterization of six mutants from our screen for meiotic mutants. These mutants show defective telomere clustering as demonstrated by mislocalization of Swi6::GFP, a heterochromatin-binding protein, and Taz1p::GFP, a telomere-specific protein. These mutants define four complementation groups and are named dot1 to dot4-defective organization of telomeres. dot3 and dot4 are allelic to mat1-Mm and mei4, respectively. Immunolocalization of Sad1, a protein associated with the spindle pole body (SPB), in dot mutants showed an elevated frequency of multiple Sad1-nuclei signals relative to wild type. Many of these Sad1 foci were colocalized with Taz1::GFP. Impaired SPB structure and function were further demonstrated by failure of spore wall formation in dot1, by multiple Pcp1::GFP signals (an SPB component) in dot2, and by abnormal microtubule organization...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Dec 1, 1990·Genome Génome / Conseil National De Recherches Canada·J Loidl
Nov 1, 1990·The Journal of Cell Biology·M P Rout, J V Kilmartin
Sep 1, 1974·Experimental Cell Research·R Egel, M Egel-Mitani
Jan 1, 1968·Molecular & General Genetics : MGG·C BreschR Egel
Oct 1, 1994·The Journal of Cell Biology·H ScherthanJ Kohli
Apr 8, 1994·Science·Y ChikashigeY Hiraoka
Apr 1, 1993·The Journal of Cell Biology·J BählerJ Kohli
Feb 3, 1999·Annual Review of Genetics·D Zickler, N Kleckner
Jan 7, 2000·Experimental Cell Research·K OkazakiO Niwa
Sep 27, 2000·Current Topics in Developmental Biology·I M Hagan, J Petersen

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Citations

Sep 23, 2008·Trends in Genetics : TIG·Mara N Stewart, Dean S Dawson
Jul 4, 2008·Annual Review of Cell and Developmental Biology·Needhi Bhalla, Abby F Dernburg
Aug 19, 2004·Journal of Cell Science·Lisa HarperW Zacheus Cande
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Daniël Van DammeDanny Geelen
Feb 20, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Eduardo Corredor, Tomás Naranjo
Oct 29, 2004·Journal of Cell Science·Teresa NiccoliMasayuki Yamamoto
Nov 18, 2004·Molecular Cell·Mark R FloryRuedi Aebersold
Jun 14, 2006·The Journal of Cell Biology·Xie TangW Zacheus Cande
Feb 8, 2005·Current Biology : CB·Immanual JosephArthur J Lustig
Jul 20, 2007·Chromosoma·Yuji ChikashigeYasushi Hiraoka
May 28, 2008·Cytogenetic and Genome Research·T Naranjo, E Corredor
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ira M HallShiv I S Grewal
Jan 28, 2003·International Journal for Parasitology·J D BarryR McCulloch
Jul 19, 2002·Yeast

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