Fission yeast Rad17 associates with chromatin in response to aberrant genomic structures

Molecular and Cellular Biology
M KaiT S Wang

Abstract

Fission yeast checkpoint protein Rad17 is required for the DNA integrity checkpoint responses. A fraction of Rad17 is chromatin bound independent of the other checkpoint proteins throughout the cell cycle. Here we show that in response to DNA damage induced by either methyl methanesulfonate treatment or ionizing radiation, increased levels of Rad17 bind to chromatin. Following S-phase stall induced by hydroxyurea or a cdc22 mutation, the chromatin-bound Rad17 progressively dissociates from the chromatin. After S-phase arrest by hydroxyurea in cds1Delta or rad3Delta cells or by replication mutants, Rad17 remains chromatin bound. Rad17 is able to complex in vivo with an Rfc small subunit, Rfc2, but not with Rfc1. Furthermore, cells with rfc1Delta are checkpoint proficient, suggesting that Rfc1 does not have a role in checkpoint function. A checkpoint-defective mutant protein, Rad17(K118E), which has similar nuclear localization to that of the wild type, is unable to bind ATP and has reduced ability in chromatin binding. Mutant Rad17(K118E) protein also has reduced ability to complex with Rfc2, suggesting that Lys(118) of Rad17 plays a role in Rad17-Rfc small-subunit complex formation and chromatin association. However, in the rad...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Nov 3, 1989·Science·L H Hartwell, T A Weinert
Aug 1, 1974·Journal of General Microbiology·D G Smith
Jan 1, 1994·Journal of Cell Science. Supplement·Y SakaM Yanagida
Dec 16, 1994·Science·L H Hartwell, M B Kastan
Jan 11, 1993·Nucleic Acids Research·M O'DonnellJ Hurwitz
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoK Matsumoto
May 23, 1998·Science·M N BoddyP Russell
Jul 11, 1998·The Journal of Biological Chemistry·A E ParkerW H Luyten
Aug 7, 1998·Molecular Biology of the Cell·D Bhaumik, T S Wang
Aug 26, 1998·Molecular and Cellular Biology·T ShimomuraK Sugimoto
Oct 6, 1998·Annual Review of Biochemistry·S Waga, B Stillman
Jan 16, 1999·Molecular and Cellular Biology·V F LiuT S Wang
Jan 23, 1999·Current Opinion in Cell Biology·N Rhind, P Russell
Feb 2, 1999·The EMBO Journal·J TurnerM O'Donnell
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G ZhangJ Hurwitz
Apr 10, 1999·Molecular Biology of the Cell·B FurnariP Russell
May 18, 1999·Molecular and Cellular Biology·J M BrondelloP Russell
Nov 24, 1999·Nature Cell Biology·R Michelson, T Weinert
Dec 10, 1999·Molecular Biology of the Cell·S G Pasion, S L Forsburg
Feb 5, 2000·Current Biology : CB·C M GreenN F Lowndes
Mar 14, 2000·The Journal of Biological Chemistry·T Bessho, A Sancar

❮ Previous
Next ❯

Citations

Nov 6, 2003·Toxicology·Markus ChristmannBernd Kaina
May 23, 2007·Nature Cell Biology·Mihoko KaiTeresa S F Wang
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasushi ShiomiToshiki Tsurimoto
Sep 16, 2003·Molecular Biology of the Cell·Rhiannon E JonesHoward D Lindsay
Jan 7, 2003·Genes & Development·Mihoko Kai, Teresa S-F Wang
Feb 5, 2002·Genes & Development·Eun-Jin Erica Hong, G Shirleen Roeder
Jul 30, 2008·Molecular and Cellular Biology·Chaitali DuttaNicholas Rhind
Sep 6, 2002·The Journal of Cell Biology·Matthew P StokesW Matthew Michael
Apr 2, 2008·Cancer Letters·Giovanni L BerettaPaola Perego
Apr 7, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasushi ShiomiToshiki Tsurimoto
Dec 25, 2012·Molecular Cell·Georgios Ioannis KarrasStefan Jentsch
Aug 29, 2006·Molecular Cell·George-Lucian MoldovanStefan Jentsch
Dec 14, 2004·Advances in Protein Chemistry·Jean Y J Wang, Sarah K Cho
Feb 18, 2004·Oncogene·David K CrockettMegan S Lim
Sep 7, 2001·Genes & Development·R T Abraham

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.