Abstract
The Rho GTPase family and their effectors are key regulators involved in many eukaryotic cell functions related to actin organization and polarity establishment. Schizosaccharomyces pombe Rho1p is essential, directly activates the (1,3)-beta-d-glucan synthase, and participates in regulation of cell wall growth and morphogenesis. Here we describe the characterization of the fission yeast Rho5p GTPase, highly homologous to Rho1p, sharing 86% identity and 95% similarity. Overexpression of the hyperactive allele rho5-G15V causes a morphological effect similar to that of rho1-G15V, but the penetrance is significantly lower, and overexpression of the dominant-negative allele rho5-T20N causes lysis like that of rho1-T20N. Importantly, overexpression of rho5(+) but no other rho genes is able to rescue the lethality of rho1Delta cells. Shutoff experiments indicated that Rho5p can replace Rho1p, but it is not as effective in maintaining cell wall integrity or actin organization. rho5(+) expression is hardly detected during log-phase growth but is induced under nutritional starvation conditions. rho5Delta cells are viable and do not display any defects during logarithmic growth. However, when rho1(+) expression is repressed during station...Continue Reading
References
Jan 10, 1991·Nature·H R BourneF McCormick
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Mar 1, 1994·Genetics·J B Keeney, J D Boeke
Jun 25, 1993·Nucleic Acids Research·S L Forsburg
Oct 6, 1997·Genes & Development·L Van Aelst, C D'Souza-Schorey
Mar 10, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K NakanoI Mabuchi
Aug 8, 1998·The Journal of Biological Chemistry·D J Mackay, A Hall
Aug 26, 1998·Yeast·J BählerJ R Pringle
Oct 6, 1998·Annual Review of Biochemistry·E CabibT Drgon
Dec 16, 1998·Gene·R A CravenA M Carr
Oct 3, 1999·Microscopy Research and Technique·M ArellanoP Pérez
Aug 3, 2000·FEBS Letters·J LiuM Balasubramanian
Nov 9, 2000·Molecular Microbiology·V MartínY Sánchez
Dec 5, 2000·Molecular Biology of the Cell·T M CalongeP Perez
Jan 12, 2001·Physiological Reviews·Y TakaiT Matozaki
Mar 16, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takahiro TouganHiroshi Nojima
Aug 6, 2002·Nature Genetics·Juan MataJürg Bähler
Nov 5, 2002·Journal of Cell Science·Kentaro NakanoIssei Mabuchi
Nov 30, 2002·Current Opinion in Microbiology·Paul K Herman
Dec 13, 2002·Nature·Sandrine Etienne-Manneville, Alan Hall
Mar 26, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kentaro NakanoIssei Mabuchi
Jun 6, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yoshito KakiharaHiroshi Nojima
Jun 11, 2003·Eukaryotic Cell·Beatriz SantosPilar Pérez
Aug 22, 2003·Genetics·Hongyan WangMohan K Balasubramanian
Feb 12, 2004·Eukaryotic Cell·Tomohiro NakamuraChikashi Shimoda
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Joseph V GrayMargaret Werner-Washburne
Nov 18, 2004·Journal of Cell Science·Virginia TajaduraYolanda Sánchez
Sep 9, 2005·FEBS Letters·Kentaro NakanoIssei Mabuchi
Oct 11, 2005·Eukaryotic Cell·Beatriz SantosPilar Pérez
Citations
May 11, 2012·Molecular Biology of the Cell·Luther W PollardMatthew Lord
Aug 24, 2013·Molecular Biology of the Cell·Yi-Hua ZhuJian-Qiu Wu
Dec 13, 2006·Eukaryotic Cell·Satoru IshiharaYoshikazu Ohya
Feb 13, 2013·BMC Microbiology·Marisa MadridJosé Cansado
Nov 21, 2014·Molecular Biology of the Cell·Ning WangJian-Qiu Wu
Aug 21, 2015·Current Opinion in Microbiology·Valeria Davì, Nicolas Minc
Feb 6, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akira DoiReiko Sugiura
Apr 17, 2015·BMC Genomics·Francisco E NicolásVictoriano Garre
Oct 31, 2006·Yeast·Patricia GarcíaYolanda Sánchez
Aug 13, 2013·Genetics·Raul A VianaPilar Pérez
Apr 26, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Julia HühnJürgen J Heinisch
Apr 17, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Pilar PérezJose Cansado
Jul 3, 2021·Cells·Jero Vicente-SolerMarisa Madrid