Fission yeast Rho5p GTPase is a functional paralogue of Rho1p that plays a role in survival of spores and stationary-phase cells

Eukaryotic Cell
Sergio A RincónP Perez

Abstract

The Rho GTPase family and their effectors are key regulators involved in many eukaryotic cell functions related to actin organization and polarity establishment. Schizosaccharomyces pombe Rho1p is essential, directly activates the (1,3)-beta-d-glucan synthase, and participates in regulation of cell wall growth and morphogenesis. Here we describe the characterization of the fission yeast Rho5p GTPase, highly homologous to Rho1p, sharing 86% identity and 95% similarity. Overexpression of the hyperactive allele rho5-G15V causes a morphological effect similar to that of rho1-G15V, but the penetrance is significantly lower, and overexpression of the dominant-negative allele rho5-T20N causes lysis like that of rho1-T20N. Importantly, overexpression of rho5(+) but no other rho genes is able to rescue the lethality of rho1Delta cells. Shutoff experiments indicated that Rho5p can replace Rho1p, but it is not as effective in maintaining cell wall integrity or actin organization. rho5(+) expression is hardly detected during log-phase growth but is induced under nutritional starvation conditions. rho5Delta cells are viable and do not display any defects during logarithmic growth. However, when rho1(+) expression is repressed during station...Continue Reading

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Citations

Feb 13, 2013·BMC Microbiology·Marisa MadridJosé Cansado
Aug 21, 2015·Current Opinion in Microbiology·Valeria Davì, Nicolas Minc
Feb 6, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akira DoiReiko Sugiura
Apr 26, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Julia HühnJürgen J Heinisch
Apr 17, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Pilar PérezJose Cansado
Jul 3, 2021·Cells·Jero Vicente-SolerMarisa Madrid

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