PMID: 6165704Jan 1, 1981Paper

FITC-dextran tracers in microcirculatory and permeability studies using combined fluorescence stereo microscopy, fluorescence light microscopy and electron microscopy

Histochemistry
N Thorball

Abstract

Coupling fluorescein-isothiocyanate to dextrans (FITC-D) extends the usefulness of the dextrans as electron microscopic tracer particles by permitting preceding fluorescence stereo microscopy and high-power light microscopy of the tissue specimens. The fate of the tracer may thus be studied in vivo during the experiment, during fixation, and during the succeeding tissue processing. A study of some simple physicochemical characteristics of the tracer, and the influence, if any, of the fixing agent are also made possible. FITC-D was found to be uncharged in the pH range from 6.5 to 8.5, more rapidly precipitated by acetone than by alcohol, and to react with glutaraldehyde and osmium tetroxide in an unknown way during tissue fixation. FITC-D with molecular weights 70,000 and 150,000 showed no signs of diffusion during tissue preparation with the methods reported in the paper, whereas FITC-D 40,000 did so to a slight degree, when the tissue was kept for several days in the fixative vehicle. Securing the preservation of the lower molecular weight FITC-Ds during tissue fixation and preparation is more difficult and the described methods are not adequate. Dextrans provoke an anaphylactic reaction in most rat strains, but are well tole...Continue Reading

References

Feb 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G I Malinin
Aug 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N Simionescu
Aug 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E A BayerM Wilchek
Mar 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G I Malinin
Jul 1, 1979·Proceedings of the Society for Experimental Biology and Medicine·C H BakerE T Sutton
Nov 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S K Ainsworth
Sep 1, 1976·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G I Malinin
Jan 1, 1975·Microvascular Research·Y Nakamura, H Wayland
Jan 1, 1976·Microvascular Research·U SchröderO Tangen
Sep 1, 1971·The Journal of Cell Biology·N Simionescu, G E Palade
May 1, 1972·The Journal of Cell Biology·N SimionescuG E Palade
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S K AinsworthM J Karnovsky
Jan 1, 1974·Advances in Carbohydrate Chemistry and Biochemistry·R L Sidebotham
Jul 1, 1967·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Rambourg
Feb 1, 1969·The Journal of Cell Biology·A RambourgC P Leblond
Jul 1, 1973·Neuroscience and Behavioral Physiology·G G Arakelov
Jan 1, 1968·Medicina Experimentalis. International Journal of Experimental Medicine·P I BrånemarkJ Lindhe
Apr 1, 1969·The Journal of Cell Biology·F Clementi, G E Palade
Nov 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M SeligmanL Katzoff
Apr 1, 1966·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C Graham, M J Karnovsky
Feb 1, 1983·Journal of Microscopy·N Thorball, J Tranum-Jensen
May 1, 1965·The Journal of Cell Biology·J H VENABLE, R COGGESHALL
Jan 28, 1955·Deutsche medizinische Wochenschrift·H SCHMIDTW HEINEN
Dec 1, 1961·The Journal of Biophysical and Biochemical Cytology·G MAJNOG I SCHOEFL

❮ Previous
Next ❯

Citations

Sep 1, 1994·Cell and Tissue Research·L J Andries, D L Brutsaert
Apr 1, 1995·Anatomy and Embryology·R N Feinberg, E Cafasso
Apr 25, 2012·Cell and Tissue Research·Adriane G OtopalikNancy H Kolodny
Nov 1, 1986·Journal of Neuroscience Methods·J C GloverJ K Jansen
Jan 1, 1991·Annals of the New York Academy of Sciences·R M Grossfeld
May 9, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kaushik Parthasarathi
Nov 7, 2009·Stroke; a Journal of Cerebral Circulation·Bo ChenPatrick D Lyden
Sep 1, 1992·Journal of Neurosurgery·K Ohata, A Marmarou
Aug 20, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Mohammed AliSujit Kumar Ghosh
Feb 1, 2011·Journal of Drug Targeting·Alexander KoshkaryevVladimir P Torchilin
Dec 13, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Cornelia E FarnumRebecca Williams
Jul 1, 1989·Acta Anaesthesiologica Scandinavica·T WetterbergH Willén
Jan 1, 1988·Glia·R M GrossfeldL C Stewart
Sep 1, 1985·The Anatomical Record·H B MikkelsenN Thorball
Jul 12, 2016·Journal of Molecular and Cellular Cardiology·Anna FreyStefan Frantz
Oct 15, 2011·Experimental Biology and Medicine·Anteneh AddisuJohn R Dietz
Aug 25, 2015·International Journal of Systematic and Evolutionary Microbiology·Sang Rim KangSang-Seob Lee
Feb 11, 2009·Analytical Chemistry·Ju Hun Yeon, Je-Kyun Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anaphylaxis

Anaphylaxis is a serious allergic reaction that is rapid in onset and may cause death.