Fitness analyses of vesicular stomatitis strains with rearranged genomes reveal replicative disadvantages

Journal of Virology
Isabel S NovellaGail W Wertz

Abstract

Gene expression of the nonsegmented negative-strand RNA viruses is determined by the position of each gene relative to that the single 3' promoter. The general order of genes among all of the viruses of the order Mononegavirales is highly conserved. In previous work we generated recombinant viruses in which the order of the three central genes of the prototypical rhabdovirus, vesicular stomatitis virus, was rearranged to all six possible permutations. While some of these viruses replicated less well than the wild type when assayed by single-step growth analyses in BSC-1 cells, others replicated as well or slightly better. In the work reported here, we used competition assays to compare the fitness of the viruses with alternative gene orders to that of the wild-type (wt) virus. We found that the relative fitness of these recombinant viruses depended on the multiplicity of infection (MOI) but not on the population size. However, during competitions at low MOI, when complementation cannot compensate for the defects of the populations with rearranged genomes, the virus with the wt gene order was always the most fit.

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Citations

Jul 5, 2011·Nature Reviews. Microbiology·Etienne Simon-Loriere, Edward C Holmes
Sep 29, 2006·Journal of Virology·Xiaodong ZhaoWayne M Sullender
Dec 10, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amber MillerStephen J Russell
Mar 20, 2014·The Journal of General Virology·Alexander T CiotaLaura D Kramer
Jun 26, 2013·Virus Research·Eric HastieValery Z Grdzelishvili
Jun 13, 2015·Virus Research·Alexander Ghanem, Karl-Klaus Conzelmann
Apr 18, 2015·Frontiers in Genetics·Kendra PeskoPaul E Turner
Aug 6, 2005·Virology·R SpringmanJ J Bull
Mar 24, 2005·The Journal of General Virology·Ashley C BanyardThomas Barrett
Mar 24, 2005·The Journal of General Virology·M D BaronT Barrett
Jun 26, 2013·G3 : Genes - Genomes - Genetics·Nicole CecchiniJames J Bull
Dec 13, 2018·Virusdisease·Belayneh GetachewRabindra Prasad Singh
May 1, 2019·Viruses·Mingzhu MeiXiaofeng Guo

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