Jun 14, 2012

Fitness landscape transformation through a single amino acid change in the rho terminator

PLoS Genetics
Peter L FreddolinoSaeed Tavazoie

Abstract

Regulatory networks allow organisms to match adaptive behavior to the complex and dynamic contingencies of their native habitats. Upon a sudden transition to a novel environment, the mismatch between the native behavior and the new niche provides selective pressure for adaptive evolution through mutations in elements that control gene expression. In the case of core components of cellular regulation and metabolism, with broad control over diverse biological processes, such mutations may have substantial pleiotropic consequences. Through extensive phenotypic analyses, we have characterized the systems-level consequences of one such mutation (rho*) in the global transcriptional terminator Rho of Escherichia coli. We find that a single amino acid change in Rho results in a massive change in the fitness landscape of the cell, with widely discrepant fitness consequences of identical single locus perturbations in rho* versus rho(WT) backgrounds. Our observations reveal the extent to which a single regulatory mutation can transform the entire fitness landscape of the cell, causing a massive change in the interpretation of individual mutations and altering the evolutionary trajectories which may be accessible to a bacterial population.

  • References40
  • Citations18

References

  • References40
  • Citations18

Citations

Mentioned in this Paper

Metabolic Process, Cellular
Gene Expression Regulation, Bacterial
Biochemical Pathway
Structure of Spinothalamic Tract of Pons
Ethanol
Exertion
Glycerin
Alkalescens-Dispar Group
Ethanol Measurement
Genome

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