Apr 25, 2020

The Bacterial Cytoskeleton Spatially Confines Functional Membrane Microdomains

BioRxiv : the Preprint Server for Biology
R. M. WagnerDaniel Lopez

Abstract

Cell membranes laterally segregate into microdomains enriched in certain lipids and scaffold proteins. Membrane microdomains modulate protein protein interactions and are essential for cell polarity, signaling and membrane trafficking. How cells organize their membrane microdomains, and the physiological importance of these microdomains, is unknown. In eukaryotes, the cortical actin cytoskeleton is proposed to act like a fence, constraining the dynamics of membrane microdomains. Like their eukaryotic counterparts, bacterial cells have functional membrane microdomains (FMMs) that act as platforms for the efficient oligomerization of protein complexes. In this work, we used the model organism Bacillus subtilis to demonstrate that FMM organization and movement depend primarily on the interaction of FMM scaffold proteins with the domains protein cargo, rather than with domain lipids. Additionally, the MreB actin-like cytoskeletal network that underlies the bacterial membrane was found to frame areas of the membrane in which FMM mobility is concentrated. Variations in membrane fluidity did not affect FMM mobility whereas alterations in cell wall organization affected FMM mobility substantially. Interference with MreB organization al...Continue Reading

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Mentioned in this Paper

Research
Cessation of Life
Cancer Progression
Biologic Development
Organ
Cardiovascular Diseases
Documented
Malignant Neoplasms
Carcinogenicity
Cancer Stem Cells

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