Five-dimensional two-photon volumetric microscopy of in-vivo dynamic activities using liquid lens remote focusing.

Biomedical Optics Express
Kayvan Forouhesh TehraniLuke J Mortensen

Abstract

Multi-photon scanning microscopy provides a robust tool for optical sectioning, which can be used to capture fast biological events such as blood flow, mitochondrial activity, and neuronal action potentials. For many studies, it is important to visualize several different focal planes at a rate akin to the biological event frequency. Typically, a microscope is equipped with mechanical elements to move either the sample or the objective lens to capture volumetric information, but these strategies are limited due to their slow speeds or inertial artifacts. To overcome this problem, remote focusing methods have been developed to shift the focal plane axially without physical movement of the sample or the microscope. Among these methods is liquid lens technology, which adjusts the focus of the lens by changing the wettability of the liquid and hence its curvature. Liquid lenses are inexpensive active optical elements that have the potential for fast multi-photon volumetric imaging, hence a promising and accessible approach for the study of biological systems with complex dynamics. Although remote focusing using liquid lens technology can be used for volumetric point scanning multi-photon microscopy, optical aberrations and the effe...Continue Reading

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Citations

Nov 14, 2019·American Journal of Physiology. Cell Physiology·Anna S NichenkoJarrod A Call
Sep 11, 2020·Optics Express·Zongyue ChengMeng Cui
Aug 28, 2020·Journal of Biophotonics·Kayvan Forouhesh TehraniLuke J Mortensen
Oct 9, 2020·Connective Tissue Research·Kayvan Forouhesh TehraniLuke J Mortensen
Mar 7, 2021·International Journal of Molecular Sciences·Anna S NichenkoJarrod A Call
Jul 6, 2021·Biomedical Optics Express·Soheil MojiriJörg Enderlein
Jul 6, 2021·Biomedical Optics Express·Antoine M ValeraR Angus Silver
Jul 17, 2021·Optics Express·Zhao JiangQiong-Hua Wang

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