PMID: 9447919Feb 3, 1998Paper

Fixation-induced shrinkage of Reissner's membrane and its potential influence on the assessment of endolymph volume

Hearing Research
A S Brunschwig, Alec N Salt

Abstract

The quantification of endolymph volume by histological techniques or by magnetic resonance (MR) microscopy requires the inner ear to be first treated with chemical fixatives. If the fixative induces soft-tissue shrinkage, it would tend to return a distended Reissner's membrane towards a straight position, since this membrane is anchored to bone at its medial and lateral edges. The goal of this study was to determine the degree of Reissner's membrane shrinkage induced by different fixation protocols to establish methods which minimize tissue shrinkage. Fragments of fresh Reissner's membrane were dissected from isolated cochleae in an artificial perilymph. Specimens were viewed with an inverted microscope during infusion of fixatives, and changes recorded on video tape. Size changes of the specimen were quantified, usually over a 20 min period. Heidenhain-Susa, a fixative which is widely used in histological studies of hydropic cochleae, caused substantial shrinkage of Reissner's membrane, decreasing the length of specimens by an average of 15.1%. Other fixation procedures induced far less shrinkage. The use of 3.1% glutaraldehyde in Hanks' balanced salt solution produced a mean length decrease of only 0.3%. The inclusion in the ...Continue Reading

References

Jan 1, 1983·Virchows Archiv. A, Pathological Anatomy and Histopathology·H BoonstraG J Fleuren
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May 1, 1980·The Journal of the Acoustical Society of America·D J Lim
May 1, 1994·Hearing Research·M M HensonG A Johnson

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Citations

Jul 1, 2011·International Journal of Otolaryngology·Daniel J Pender
Apr 10, 2012·PloS One·Christoph RauClaus-Peter Richter
Oct 18, 2014·AJNR. American Journal of Neuroradiology·A van der JagtB M Verbist
Mar 9, 2017·Journal of Otolaryngology - Head & Neck Surgery = Le Journal D'oto-rhino-laryngologie Et De Chirurgie Cervico-faciale·Robert W KochSumit K Agrawal
Dec 10, 2016·Journal of Microscopy·M ElfarnawanyH M Ladak
Feb 24, 2001·The Annals of Otology, Rhinology, and Laryngology·Y ShinomoriR S Kimura
Mar 15, 2018·Frontiers in Neuroscience·Lejo Johnson ChackoAnneliese Schrott-Fischer

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