Fixed archival tissue. Purify DNA and primers for good PCR yield!

Molecular Biotechnology
G WeirichH Brauch

Abstract

The analysis of archival formalin-fixed, paraffin-embedded tissue samples becomes increasingly important for molecular biology studies. As fixation and paraffin-embedding cause alterations of proteins and nucleic acids, archival sources of DNA must be handled with care. To address the need for specificity and reproducibility, we developed an improved protocol for semi-automated DNA extraction adapted to fixed, embedded tissue samples, and a PCR approach using HPLC-purified primers.

References

Mar 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Ben-EzraA Wu
Jan 1, 1988·The Journal of Experimental Medicine·D K ShibataW J Martin

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Citations

Feb 5, 2010·Forensic Science International. Genetics·Kristin DuvalChantal J Frégeau
Mar 12, 2004·Molecular Phylogenetics and Evolution·A Bucklin, L D Allen
Feb 7, 2006·Biosensors & Bioelectronics·Avraham Rasooly, James Jacobson
Oct 12, 2002·American Journal of Medical Genetics·Shawnia ForresterVirginia E Kimonis
Mar 30, 2001·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·A BergerB Kofler
Jun 30, 2000·Human Mutation·M L NickersonL S Schmidt

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