Flagellar Basal Body Structural Proteins FlhB, FliM, and FliY Are Required for Flagellar-Associated Protein Expression in Listeria monocytogenes

Frontiers in Microbiology
Changyong ChengHouhui Song

Abstract

Listeria monocytogenes is a food-associated bacterium that is responsible for food-related illnesses worldwide. In the L. monocytogenes EGD-e genome, FlhB, FliM, and FliY (encoded by lmo0679, lmo0699, and lmo0700, respectively) are annotated as putative flagella biosynthesis factors, but their functions remain unknown. To explore whether FlhB, FliM, and FliY are involved in Listeria flagella synthesis, we constructed flhB, fliM, fliY, and other flagellar-related gene deletion mutants using a homologous recombination strategy. Then, we analyzed the motility, flagella synthesis, and protein expression of these mutant strains. Motility and flagella synthesis were completely abolished in the absence of flhB, fliM, or fliY. These impaired phenotypes were fully restored in the complemented strains CΔflhB, CΔfliM, and CΔfliY. The transcriptional levels of flagellar-related genes, including flaA, fliM, fliY, lmo0695, lmo0698, fliI, and fliS, were downregulated markedly in the absence of flhB, fliM, or fliY. Deletion of flhB resulted in the complete abolishment of FlaA expression, while it decreased FliM and FliY expression. The expression of FlaA was abolished completely in the absence of fliM or fliY. No significant changes were found...Continue Reading

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Citations

Feb 11, 2020·Frontiers in Microbiology·Martin Laage Kragh, Lisbeth Truelstrup Hansen
Feb 16, 2021·Frontiers in Microbiology·Constanza MelianGraciela Margarita Vignolo
Jul 24, 2021·Trends in Biochemical Sciences·Haidai HuNicholas M I Taylor

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Methods Mentioned

BETA
transmission electron microscopy
motility assay
PCR
GTPase
affinity column chromatography
Assay
PCRs
electrophoresis

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