Flagellar Stators Stimulate c-di-GMP Production by Pseudomonas aeruginosa

Journal of Bacteriology
Amy E BakerGeorge A O'Toole

Abstract

Flagellar motility is critical for surface attachment and biofilm formation in many bacteria. A key regulator of flagellar motility in Pseudomonas aeruginosa and other microbes is cyclic diguanylate (c-di-GMP). High levels of this second messenger repress motility and stimulate biofilm formation. c-di-GMP levels regulate motility in P. aeruginosa in part by influencing the localization of its two flagellar stator sets, MotAB and MotCD. Here, we show that while c-di-GMP can influence stator localization, stators can in turn impact c-di-GMP levels. We demonstrate that the swarming motility-driving stator MotC physically interacts with the transmembrane region of the diguanylate cyclase SadC. Furthermore, we demonstrate that this interaction is capable of stimulating SadC activity. We propose a model by which the MotCD stator set interacts with SadC to stimulate c-di-GMP production under conditions not permissive to motility. This regulation implies a positive-feedback loop in which c-di-GMP signaling events cause MotCD stators to disengage from the motor; then disengaged stators stimulate c-di-GMP production to reinforce a biofilm mode of growth. Our studies help to define the bidirectional interactions between c-di-GMP and the f...Continue Reading

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Citations

May 22, 2019·Journal of Bacteriology·Kimberley A LewisDeborah A Hogan
Jul 4, 2019·Chemistry : a European Journal·Sina Rütschlin, Thomas Böttcher
Apr 25, 2020·Environmental Microbiology·Guang-Lei MaZhao-Xun Liang
Jul 22, 2020·Annual Review of Microbiology·Alan J CollinsGeorge A O'Toole
Apr 10, 2019·Journal of Bacteriology·Daniel B Kearns
May 31, 2019·Proceedings of the National Academy of Sciences of the United States of America·Navish WadhwaHoward C Berg
Jan 22, 2020·Nature Reviews. Microbiology·Yves F Dufrêne, Alexandre Persat
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Apr 3, 2021·Microorganisms·Helga Fernández-LlamosasManuel Carmona
Apr 21, 2021·Proceedings of the National Academy of Sciences of the United States of America·Navish WadhwaHoward C Berg

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