Flagellin is required for salmonella-induced expression of heat shock protein Hsp25 in intestinal epithelium

American Journal of Physiology. Gastrointestinal and Liver Physiology
Elaine O PetrofEugene B Chang

Abstract

Flagellin is a bacterial protein responsible for activation of Toll-like receptor 5 (TLR5), which we hypothesize is involved in Salmonella's induction of cytoprotective heat shock proteins in intestinal epithelial cells. Flagellin induces the cytoprotective heat shock protein Hsp25 in different intestinal epithelial cell lines and in mouse intestine. Flagellin induces Hsp25 expression in a time-dependent manner in vitro. This effect is transcriptional, as confirmed by luciferase reporter assays and actinomycin D treatment. In addition, Hsp25 induction requires p38 MAPK activation and is only observed when flagellin is added to the basolateral side of polarized intestinal epithelial cells, consistent with the known location of TLR5. Flagellin-mediated Hsp25 induction is associated with increased protective effects against oxidant stress, an effect that is at least partially mediated by p38 MAPK. Use of small interfering RNA against Hsp25 demonstrates that flagellin-mediated protection against oxidant stress is to some degree mediated through Hsp25 induction. This suggests that, by protecting against oxidant injury, the induction of Hsp25 expression by flagellin may contribute to intestinal homeostasis. In a coculture cell model ...Continue Reading

References

Dec 1, 1990·Inflammation·M B GrishamE A Deitch
Mar 1, 1988·The American Journal of Physiology·K M CarrollE B Chang
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R H WhiteheadP S Jat
Apr 1, 1994·Digestive Diseases and Sciences·P KrugliakT Y Ma
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A T GewirtzJ L Madara
Apr 19, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yimin YuAndrew T Gewirtz
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hui ZengAndrew S Neish
Nov 25, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Keishi KojimaEugene B Chang
Nov 6, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Donna L ArvansEugene B Chang

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Citations

Feb 18, 2016·Nutrition Reviews·Marie-Edith Arnal, Jean-Paul Lallès
Feb 27, 2009·Mucosal Immunology·M Vijay-Kumar, A T Gewirtz
Sep 2, 2017·Experimental & Molecular Medicine·Irshad A HajamJohn Hwa Lee
Apr 3, 2009·Journal of Agricultural and Food Chemistry·M Martínez-GomarizG Préstamo

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