Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells.

Infection and Immunity
Xin ZhouJ B Kaper

Abstract

The type III secretion system (TTSS) of enteropathogenic Escherichia coli (EPEC) has been associated with the ability of these bacteria to induce secretion of proinflammatory cytokines, including interleukin-8 (IL-8), in cultured epithelial cells. However, the identity of the effector molecule directly involved in this event is unknown. In this study, we determined that the native flagellar filament and its flagellin monomer are activators of IL-8 release in T84 epithelial cells. Supernatants of wild-type EPEC strain E2348/69 and its isogenic mutants deficient in TTSS (escN) and in production of intimin (eae), grown in Luria-Bertani broth, elicited similar amounts of IL-8 secretion by T84 cells. In contrast, supernatants of EPEC fliC mutants and of B171, a nonflagellated EPEC strain, were defective in inducing IL-8 release, a phenotype that was largely restored by complementation of the fliC gene in the mutant lacking flagella. Purified flagella from E. coli K-12, EPEC serotypes H6 and H34, and enterohemorrhagic E. coli serotype H7 all induced IL-8 release in T84 cells. Induction of IL-8 by purified flagella or His-tagged FliC from EPEC strain E2348/69 was dose dependent and was blocked by a polyclonal anti-H6 antibody. Finally...Continue Reading

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Citations

May 4, 2007·Inflammatory Bowel Diseases·Nathalie Rolhion, Arlette Darfeuille-Michaud
Apr 6, 2004·Advanced Drug Delivery Reviews·V K ViswanathanGail Hecht
Sep 20, 2006·Infection and Immunity·Shelley N LuckElizabeth L Hartland
Nov 23, 2006·Infection and Immunity·Theodore S Steiner
May 23, 2006·Infection and Immunity·Priscilla A Johanesen, Michael B Dwinell
Dec 23, 2003·Infection and Immunity·Xin ZhouJames B Kaper
Dec 25, 2004·Infection and Immunity·Alfredo G TorresJames B Kaper
Oct 22, 2005·Infection and Immunity·M Chelsea LaneHarry L T Mobley
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Feb 4, 2010·PLoS Pathogens·Chen NadlerIlan Rosenshine
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May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Erika C ClaudBobby J Cherayil
Mar 26, 2009·Nihon saikingaku zasshi. Japanese journal of bacteriology·Sunao Iyoda
Jul 6, 2010·Microbial Pathogenesis·Abdullah F Al-SayeqhIan F Connerton
Oct 13, 2015·International Journal of Medical Microbiology : IJMM·Gabriel CabreraMarina S Palermo

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