Flagellin of enteropathogenic Escherichia coli stimulates interleukin-8 production in T84 cells.

Infection and Immunity
Xin ZhouJ B Kaper


The type III secretion system (TTSS) of enteropathogenic Escherichia coli (EPEC) has been associated with the ability of these bacteria to induce secretion of proinflammatory cytokines, including interleukin-8 (IL-8), in cultured epithelial cells. However, the identity of the effector molecule directly involved in this event is unknown. In this study, we determined that the native flagellar filament and its flagellin monomer are activators of IL-8 release in T84 epithelial cells. Supernatants of wild-type EPEC strain E2348/69 and its isogenic mutants deficient in TTSS (escN) and in production of intimin (eae), grown in Luria-Bertani broth, elicited similar amounts of IL-8 secretion by T84 cells. In contrast, supernatants of EPEC fliC mutants and of B171, a nonflagellated EPEC strain, were defective in inducing IL-8 release, a phenotype that was largely restored by complementation of the fliC gene in the mutant lacking flagella. Purified flagella from E. coli K-12, EPEC serotypes H6 and H34, and enterohemorrhagic E. coli serotype H7 all induced IL-8 release in T84 cells. Induction of IL-8 by purified flagella or His-tagged FliC from EPEC strain E2348/69 was dose dependent and was blocked by a polyclonal anti-H6 antibody. Finally...Continue Reading


Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A E JerseJ B Kaper
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G JarvisJ B Kaper
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T K McDanielJ B Kaper
Dec 12, 1994·Archives of Internal Medicine·J R MillerR L Guerrant
Oct 1, 1995·Molecular Microbiology·J A Girón
May 1, 1996·Infection and Immunity·T E WaddellC L Gyles
Apr 1, 1997·Annals of Internal Medicine·L SlutskerP M Griffin
Nov 14, 1997·The American Journal of Physiology·S D SavkovicG Hecht
Jan 10, 1998·Infection and Immunity·M FeldmanA Prince
Feb 11, 1998·Clinical Microbiology Reviews·J P Nataro, J B Kaper
Apr 21, 1999·Current Opinion in Cell Biology·T P Garrington, G L Johnson
Oct 26, 1999·Infection and Immunity·C M ThorpeD W Acheson
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Mar 10, 2001·The Journal of Clinical Investigation·B P McNamaraG Hecht
Apr 12, 2001·Cellular Microbiology·J K CraneM S Donnenberg
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A T GewirtzJ L Madara
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·F SierroJ C Sirard
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Mar 14, 2002·International Journal of Medical Microbiology : IJMM·Brendan Kenny

❮ Previous
Next ❯


May 4, 2007·Inflammatory Bowel Diseases·Nathalie Rolhion, Arlette Darfeuille-Michaud
Apr 6, 2004·Advanced Drug Delivery Reviews·V K ViswanathanGail Hecht
Sep 20, 2006·Infection and Immunity·Shelley N LuckElizabeth L Hartland
Nov 23, 2006·Infection and Immunity·Theodore S Steiner
May 23, 2006·Infection and Immunity·Priscilla A Johanesen, Michael B Dwinell
Dec 23, 2003·Infection and Immunity·Xin ZhouJames B Kaper
Dec 25, 2004·Infection and Immunity·Alfredo G TorresJames B Kaper
Oct 22, 2005·Infection and Immunity·M Chelsea LaneHarry L T Mobley
Nov 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Robert AdamoAlice Prince
Feb 4, 2010·PLoS Pathogens·Chen NadlerIlan Rosenshine
Mar 22, 2014·The Journal of General and Applied Microbiology·Naoki SudoYasuhiko Sekine
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Erika C ClaudBobby J Cherayil
Mar 26, 2009·Nihon saikingaku zasshi. Japanese journal of bacteriology·Sunao Iyoda
Jul 6, 2010·Microbial Pathogenesis·Abdullah F Al-SayeqhIan F Connerton
Oct 13, 2015·International Journal of Medical Microbiology : IJMM·Gabriel CabreraMarina S Palermo

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.