Flagellin polymerisation control by a cytosolic export chaperone

Journal of Molecular Biology
Frederic AuvrayColin Hughes

Abstract

Assembly of the long helical filament of the bacterial flagellum requires polymerisation of ca 20,000 flagellin (FliC) monomeric subunits into the growing structure extending from the cell surface. Here, we show that export of Salmonella flagellin is facilitated specifically by a cytosolic protein, FliS, and that FliS binds to the FliC C-terminal helical domain, which contributes to stabilisation of flagellin subunit interactions during polymerisation. Stable complexes of FliS with flagellin were assembled efficiently in vitro, apparently by FliS homodimers binding to FliC monomers. The data suggest that FliS acts as a substrate-specific chaperone, preventing premature interaction of newly synthesised flagellin subunits in the cytosol. Compatible with this view, FliS was able to prevent in vitro polymerisation of FliC into filaments.

References

Oct 20, 1991·Journal of Molecular Biology·F VondervisztK Namba
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Sep 5, 1989·Journal of Molecular Biology·F VondervisztK Namba
Sep 14, 1972·Journal of Molecular Biology·E J O'Brien, P M Bennett
Feb 4, 1969·Biochimica Et Biophysica Acta·K WakabayashiS Asakura
Apr 1, 1996·Molecular Microbiology·P WattiauG R Cornelis
Jan 1, 1996·Molecular Microbiology·S I Aizawa
Apr 1, 1996·Molecular Microbiology·B J Stewart, L L McCarter
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y Mimori-KiyosueK Namba
Feb 1, 1997·Quarterly Reviews of Biophysics·K Namba, F Vonderviszt
Nov 2, 1999·Journal of Molecular Biology·S HondaK Namba
Apr 29, 2000·Trends in Microbiology·J C Bennett, C Hughes
Jun 3, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·H C Berg
Oct 1, 1964·Journal of Molecular Biology·S ASAKURAT IINO

❮ Previous
Next ❯

Citations

Dec 28, 2004·Nature Structural & Molecular Biology·Calvin K YipNatalie C J Strynadka
Aug 16, 2002·Molecular Microbiology·Robin M Delahay, Gad Frankel
May 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ariel J BlockerSusan M Lea
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gert BangeIrmgard Sinning
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Katsumi ImadaKeiichi Namba
Feb 19, 2010·The Journal of Biological Chemistry·Marcel R de ZoeteJos P M van Putten
Mar 24, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hideyuki MatsunamiKeiichi Namba
Aug 29, 2006·Journal of Bacteriology·Björn TitzPeter Uetz
Nov 13, 2012·Journal of Bacteriology·Sampriti MukherjeeDaniel B Kearns
Feb 4, 2003·Journal of Bacteriology·Lee Kroos, Janine R Maddock
Apr 3, 2003·Journal of Bacteriology·Takanori HiranoRobert M Macnab
Feb 6, 2004·Journal of Bacteriology·Rita ZilhãoAdriano O Henriques
Mar 4, 2006·Journal of Bacteriology·Jonathan FryeKelly T Hughes
Dec 26, 2002·Annual Review of Biochemistry·Howard C Berg
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Tohru Minamino, Keiichi Namba
Jun 28, 2008·PLoS Pathogens·Christian LorenzDaniela Büttner
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lewis D B EvansColin Hughes
Mar 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Joanne ThomasColin Hughes
Sep 25, 2014·Annual Review of Genetics·Sampriti Mukherjee, Daniel B Kearns
Sep 1, 2015·Frontiers in Cellular and Infection Microbiology·Lu WangQingpi Yan
Jan 19, 2012·FEMS Microbiology Reviews·Ross E Dalbey, Andreas Kuhn
May 6, 2003·Annual Review of Microbiology·Robert M Macnab
Aug 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nandish KhanraCharalampos G Kalodimos
Jan 26, 2017·Cell Reports·Bin NiVictor Sourjik
Mar 24, 2016·FEBS Letters·Ráchel SajóFerenc Vonderviszt
Aug 28, 2007·Biochimica Et Biophysica Acta·Dmitry Apel, Michael G Surette
Feb 24, 2018·Frontiers in Cellular and Infection Microbiology·Chandrani DasSupreet Saini
Oct 25, 2017·Journal of Bacteriology·Ashley N HallDaniel B Kearns
Oct 17, 2006·Nature Reviews. Microbiology·Guy R Cornelis
Jan 15, 2014·Journal of Bacteriology·Anna GalevaAlla S Kostyukova
Mar 25, 2011·Protein Science : a Publication of the Protein Society·Inoka C Perera, Anne Grove
Jun 5, 2007·Molecular Microbiology·Satoshi ShibataShin-Ichi Aizawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.