PMID: 7501641Jan 1, 1995

Flagellum-mediated adhesion of Trypanosoma congolense to bovine aorta endothelial cells

Parasitology Research
A Hemphill, C A Ross

Abstract

We studied the interaction between Trypanosoma congolense and bovine aorta endothelial (BAE) cell monolayers. Our findings suggest that trypanosomes adhere predominantly to the flattened, peripheral cell surface domains as well as to filamentous endothelial outgrowths that are present during in vitro cultivation in non-confluent monolayers. Adhesion is mediated exclusively by the flagellum in a distinct geometrical order with respect to the flagellar cytoskeleton. Thus, it is possible to define exactly the trypanosomal cell surface domain involved in the attachment process. After 24-48 h of cultivation on monolayers, trypanosomes start to develop short, filopodia-like flagellar protrusions, which serve as additional elements in assisting parasite attachment. Small filaments (3-5 nm) also serve as cross-links between flagellar and endothelial cell surface membranes. Lectin-gold labeling shows that these cross-links contain sialic acid residues. In vitro assays confirm that sialic acid is involved in the adhesion process, whereas the extracellular matrix (ECM) proteins fibronectin, collagen, laminin and vitronectin are not. The presence of T. congolense exhibits a mitogenic effect on BAE cells.

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Citations

Feb 1, 1996·Tropical Animal Health and Production·R BoidA G Luckins
Sep 20, 2012·Cell Biochemistry and Biophysics·Patricia Ponce de LeónB Riquelme
Feb 19, 2002·Trends in Parasitology·John D Lonsdale-Eccles, Dennis J Grab
Feb 1, 1996·Parasitology·A HemphillH Kaufmann
Mar 9, 2010·PLoS Neglected Tropical Diseases·Virginie CoustouThéo Baltz
Jun 17, 2014·Nature Reviews. Microbiology·Gerasimos Langousis, Kent L Hill
Nov 26, 2004·The Journal of Parasitology·Dennis J GrabKwang Sik Kim
Nov 5, 1999·Annual Review of Microbiology·K Gull
Jun 14, 2016·Pathology Research International·Alpidio A Boada-SucreArmando Reyna-Bello
May 16, 2019·Open Biology·Sara Silva PereiraLuisa M Figueiredo
Jul 25, 2014·The Journal of Veterinary Medical Science·Keisuke SuganumaNoboru Inoue
Sep 10, 2009·Revista Do Instituto De Medicina Tropical De São Paulo·P Ponce de LeónJuana Valverde
Oct 2, 2018·Emerging Topics in Life Sciences·Richard McCullochKeith Matthews
Nov 30, 2018·Journal of Helminthology·P Ponce de LeónB Riquelme
Feb 2, 2019·Current Protocols in Microbiology·Chris KayWendy Gibson

Related Concepts

Adhesiveness
Metazoa
Ascending Aorta Structure
Bos indicus
Cell Division Phases
Zyderm
Endothelium, Vascular
Fibronectins
Flagella
Laminin M

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