Flagging performance of the Sysmex XN2000 haematology analyser

International Journal of Laboratory Hematology
M SchoorlJ van Pelt

Abstract

With the introduction of the Sysmex XN haematology analyser, the white blood cell differentiation channel (WDF) and abnormal cell detection channel (WPC) have been added with algorithms for flagging blasts and abnormal or atypical lymphocytes. In this study, 2011 samples were evaluated on a Sysmex XN2000 analyser and microscopically reviewed using a CellaVision DM96 digital microscope. A reference group of apparently healthy blood donors (n = 262) demonstrated in only three samples a positive suspect flag, which could not be confirmed microscopically. Positive WBC suspect flags were demonstrated in 3% of the 2011 samples. From the 55 samples with positive WBC suspect flags, an automatic reflex test was performed within the WPC. The WPC reflex test resulted in 10× Blast?, 15× Abnormal lymph? and 15× Atypical lymph? flags, which could be confirmed microscopically in 33% of these cases. A negative flagging was demonstrated in 15 cases. Microscopic evaluation demonstrated no abnormalities in these 15 cases. However, laboratory technicians also reported the presence of abnormal lymphocytes in 158 samples without an Abnormal lymph? flag. In conclusion, the combined use of WDF and WPC resulted in a reduction of approximately 25% of th...Continue Reading

References

Jul 19, 2005·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·P W BarnesUNKNOWN international consensus group for hematology
Aug 2, 2012·Journal of Clinical Pathology·Carol BriggsSamuel J Machin
Feb 13, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mari KonoKunihiro Funakoshi

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Citations

May 1, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Wesley JongbloedRoger K Schindhelm
Sep 1, 2017·Archives of Pathology & Laboratory Medicine·Oh Joo KweonHye Ryoun Kim
Sep 28, 2017·Journal of Clinical Laboratory Analysis·Elise Schapkaitz, Phoyisile Khoza
Dec 10, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Michela SeghezziSabrina Buoro
Oct 2, 2020·International Journal of Laboratory Hematology·Siska BlommeChristine Van Laer

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