PMID: 11934494Apr 6, 2002Paper

FlaK of the archaeon Methanococcus maripaludis possesses preflagellin peptidase activity

FEMS Microbiology Letters
Sonia L Bardy, K F Jarrell

Abstract

Archaeal flagellins are initially synthesized as preflagellins with a short, positively charged leader peptide, which is cleaved prior to the incorporation of the mature flagellins into the filament. While preflagellin peptidase activity had previously been detected in methanogen membranes, the enzyme responsible for this activity had not been identified. We show here that FlaK of Methanococcus maripaludis has preflagellin peptidase activity. In an in vitro preflagellin peptidase assay, Escherichia coli membranes overexpressing Methanococcus voltae preflagellin FlaB2 (as substrate) were combined with E. coli membranes overexpressing M. maripaludis FlaK (as enzyme). Cleavage of the preflagellin was demonstrated by immunoblotting using antibody to FlaB2 and detection of a faster migrating cross-reactive species. This activity required detergent in the assay, and was not detected in membranes previously heated to 95 degrees C. This is the first reported identification of the preflagellin peptidase, and aside from the flagellins, this is the first assignment of function to a gene involved in archaeal flagellation.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1991·Journal of Bacteriology·M L Kalmokoff, K F Jarrell
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·D N Nunn, S Lory
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Mar 1, 1994·Journal of Bacteriology·J KoniskyP Haney
Sep 1, 1996·Journal of Bacteriology·K F JarrellA S Kostyukova
Oct 24, 1997·Science·R L TatusovD J Lipman
Mar 27, 1998·Molecular Microbiology·O FrancetićA P Pugsley
Apr 14, 1999·Molecular Microbiology·S V AlbersA J Driessen
Jan 8, 2000·The Journal of Biological Chemistry·C F LaPointe, R K Taylor
Mar 10, 2001·Trends in Microbiology·J Eichler, R Moll
Mar 16, 2001·FEMS Microbiology Reviews·N A ThomasK F Jarrell

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Citations

Jun 7, 2006·Nature Reviews. Microbiology·Sonja-Verena AlbersArnold J M Driessen
Apr 17, 2007·Nature Reviews. Microbiology·Mladen TomichDavid H Figurski
May 8, 2008·Nature Reviews. Microbiology·Ken F Jarrell, Mark J McBride
Aug 20, 2010·Archaea : an International Microbiological Journal·Ken F JarrellJerry Eichler
Jun 2, 2004·Journal of Molecular Microbiology and Biotechnology·Sonia L BardyKen F Jarrell
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Sandy Y M NgKen F Jarrell
Feb 5, 2014·Archives of Microbiology·Divya B NairKen F Jarrell
Jul 31, 2010·Archaea : an International Microbiological Journal·Albert F EllenSonja-Verena Albers
Aug 12, 2014·Extremophiles : Life Under Extreme Conditions·Anna-Lena HencheSonja-Verena Albers
Jun 19, 2003·Journal of Bacteriology·Sonja-Verena AlbersArnold J M Driessen
Oct 4, 2003·Journal of Bacteriology·Sandy Y M Ng, Ken F Jarrell
Apr 10, 2007·Journal of Bacteriology·Zalán SzabóArnold J M Driessen
Jul 4, 2007·BMC Evolutionary Biology·Elie DesmondSimonetta Gribaldo
Jul 16, 2008·Journal of Bacteriology·Sandy Y M NgKen F Jarrell
Dec 4, 2009·Extremophiles : Life Under Extreme Conditions·Albert F EllenArnold J M Driessen
Apr 7, 2010·Journal of Bacteriology·Manuela TripepiMechthild Pohlschröder
Apr 7, 2009·Extremophiles : Life Under Extreme Conditions·Sonja-Verena Albers, Mecky Pohlschröder
Nov 2, 2002·Molecular Microbiology·Nikhil A ThomasKen F Jarrell
Jan 27, 2011·Biochemical Society Transactions·Abhrajyoti Ghosh, Sonja-Verena Albers
Mar 7, 2013·Molecular Microbiology·Kerstin LassakSonja-Verena Albers
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Sonia L BardyKen F Jarrell
Mar 8, 2003·Microbiology·Sonia L BardyKen F Jarrell
May 17, 2011·Nature Reviews. Microbiology·Sonja-Verena Albers, Benjamin H Meyer
Nov 16, 2011·Molecular Microbiology·Kerstin LassakSonja-Verena Albers
Feb 24, 2015·Frontiers in Microbiology·Sonja-Verena Albers, Ken F Jarrell
Dec 2, 2017·Molecular Microbiology·Paushali ChaudhurySonja-Verena Albers
Feb 21, 2004·FEMS Microbiology Reviews·Mechthild PohlschröderR Wesley Rose

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