PMID: 7525949Aug 1, 1994Paper

Flash photolysis studies of the localization of calcium release sites in rat parotid isolated acinar cells

The Journal of Physiology
A A Hassoni, P T Gray

Abstract

1. The temporal relationship between cytosolic free Ca2+ concentration ([Ca2+]i) and activation of membrane current responses in single rat parotid acinar cells has been examined. Activation of muscarinic receptors by carbachol (CCh) at -40 mV (midway between EK and ECl under our experimental conditions) frequently evoked biphasic current responses, application of 2 microM CCh leading to rapid activation of an inward current followed by a slower outward current. 2. Photochemical release of inositol 1,4,5-trisphosphate (InsP3), from 'caged' InsP3, by a brief near-UV flash, evoked similar biphasic current responses at -40 mV. In contrast, elevation of [Ca2+]i by photolysis of the caged calcium compound nitr-5 at -40 mV activated only monophasic current responses. 3. These results can be explained by a model in which the InsP3-sensitive Ca2+ release sites are localized at the luminal pole of the cell, combined with a relative preponderance of Ca(2+)-activated Cl- channels at that pole, and a relative preponderance of Ca(2+)-activated K+ channels at the basal end.

Citations

Jun 9, 2000·Annual Review of Physiology·J F Kidd, P Thorn
Jul 27, 1999·Neuropathology and Applied Neurobiology·A A Hassoni, M J Cullen
Oct 10, 1997·Molecular Reproduction and Development·H IgarashiK Doi
Oct 1, 1994·Pflügers Archiv : European journal of physiology·A A Hassoni, P T Gray
Jun 1, 1996·Current Opinion in Neurobiology·J M Nerbonne

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