PMID: 10022May 30, 1975

Flash photometric experiments on the photochemical cycle of bacteriorhodopsin

Biophysics of Structure and Mechanism
N A Dencher, M Wilms

Abstract

The photochemical reaction cycle of bacteriorhodopsin was investigated by means of flash photometric methods. Three different intermediates with absorption maxima at about 630 nm, 411 nm, and 646 nm could be detected. Kinetic data of the occurrence of these intermediates were obtained from isolated purple membrane in different mediums and from intact halobacteria. An activation energy of 14.1 +/- 0.4 kcal-mol-1 and of about 19 kcal-mol-1 for formation of bacteriorhodopsin 411 and of bacteriorhodopsin 565, resp., was calculated. pH-changes in the medium caused by the reaction cycle of bacteriorhodopsin were detected by use of the pH-indicator bromocresol green.

References

Jan 1, 1974·Journal of Supramolecular Structure·W Stoeckenius, R H Lozier
Oct 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D Oesterhelt, W Stoeckenius
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A Danon, W Stoeckenius
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Citations

Apr 27, 2001·Biochemical and Biophysical Research Communications·C ZscherpJoachim Heberle
Dec 22, 1976·Biophysics of Structure and Mechanism·R KorensteinS R Caplan
Jun 29, 1977·Biophysics of Structure and Mechanism·W SperlingN A Dencher
Apr 21, 1977·Biophysics of Structure and Mechanism·E Hildebrand
Nov 27, 1978·Biophysics of Structure and Mechanism·K RosenheckS R Caplan
Oct 1, 1988·Journal of Bioenergetics and Biomembranes·M A SlifkinS R Caplan
Sep 14, 1977·The Journal of Membrane Biology·S B HwangW Stoeckenius
Aug 1, 1979·Biophysics of Structure and Mechanism·M A SlifkinS R Caplan
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Dec 10, 2009·The Journal of Physical Chemistry. B·Andrei K Dioumaev, J K Lanyi
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E BambergM P Heyn
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Marinetti, D Mauzerall
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Related Concepts

Bacteriorhodopsins
Carotenoids
Halobacterium
Hydrogen-Ion Concentration
Photochemistry
Spectrophotometry

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