Flavin nucleotide metabolism in plants: monofunctional enzymes synthesize fad in plastids.

The Journal of Biological Chemistry
Francisco J SandovalSanja Roje

Abstract

FAD synthetases (EC 2.7.7.2) catalyze biosynthesis of FAD from FMN and ATP. Monofunctional FAD synthetases are known to exist in mammals and yeast; bifunctional enzymes also catalyzing phosphorylation of riboflavin to FMN are known to exist in bacteria. Previously known eukaryotic enzymes with FAD synthetase activity have no sequence similarity to prokaryotic enzymes with riboflavin kinase and FAD synthetase activities. Proteins homologous to bacterial bifunctional FAD synthetases, yet shorter and lacking amino acid motifs at the C terminus, were found by bioinformatic analyses in vascular plant genomes, suggesting that plants contain a type of FAD synthetase previously known to exist only in prokaryotes. The Arabidopsis thaliana genome encodes two of such proteins. Both proteins, which we named AtRibF1 and AtRibF2, carry N-terminal extensions with characteristics of organellar targeting peptides. AtRibF1 and AtRibF2 cDNAs were cloned by reverse transcription-PCR. Only FAD synthetase activity was detected in the recombinant enzymes produced in Escherichia coli. FMN and ATP inhibited both enzymes. Kinetic parameters of AtRibF1 and AtRibF2 for the two substrates were similar. Confocal microscopy of protoplasts transformed with en...Continue Reading

References

Dec 30, 1992·Biochemical and Biophysical Research Communications·K R FuchsD A Bernlohr
Apr 1, 1990·Archives of Biochemistry and Biophysics·Y YamadaD B McCormick
Mar 10, 1970·The Journal of Vitaminology·H MitsudaF Kawai
Mar 10, 1970·The Journal of Vitaminology·H MistudaF Kawai
Apr 1, 1982·Archives of Biochemistry and Biophysics·E M Taga, R L Van Etten
Sep 1, 1993·Archives of Biochemistry and Biophysics·M BarileE Quagliariello
May 1, 1997·Biochemistry and Molecular Biology International·J M GranjeiroH Aoyama
Jul 21, 2000·European Journal of Biochemistry·M BarileS Passarella
Jul 14, 2001·Antimicrobial Agents and Chemotherapy·G ClareboutR Leclercq
May 7, 2002·Trends in Plant Science·Mansour KarimiAnn Depicker
Sep 19, 2002·The Journal of Biological Chemistry·Sanja RojeAndrew D Hanson
Feb 22, 2003·Journal of Molecular Biology·Stefanie BauerStefan Steinbacher
Apr 2, 2003·The Journal of Biological Chemistry·Eoin P QuinlivanAndrew D Hanson
Jun 1, 1957·Nature·K V GIRIN A RAO
Oct 1, 1958·Biochimica Et Biophysica Acta·C DELUCA, N O KAPLAN
May 1, 1960·The Biochemical Journal·K V GIRIS A KUMAR
Mar 10, 1965·The Journal of Vitaminology·H MITSUDAF KAWAI
Aug 28, 2004·Plant Physiology and Biochemistry : PPB·Noel A Tejera GarcíaCarmen Lluch
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Sep 27, 2005·The Journal of Biological Chemistry·Francisco J Sandoval, Sanja Roje
Apr 29, 2006·Biochemical and Biophysical Research Communications·Carmen BrizioMaria Barile
Oct 20, 2006·Protein Expression and Purification·Michele GalluccioMaria Barile

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Citations

Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Oct 18, 2011·The Journal of Biological Chemistry·Renu RawatSanja Roje
Dec 14, 2011·Stem Cells and Development·Selvan Christyraj Johnson Retnaraj SamuelSivassubramaniam Sudhakar
Aug 24, 2012·Journal of Experimental Botany·Svetlana GerdesAndrew D Hanson
Apr 17, 2012·Plant & Cell Physiology·Takanori MarutaShigeru Shigeoka
Jan 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Beatriz HerguedasJuan A Hermoso
Jun 8, 2011·Microbiology and Molecular Biology Reviews : MMBR·Charles A Abbas, Andriy A Sibirny
Oct 20, 2010·BMC Evolutionary Biology·Inmaculada YruelaBruno Contreras-Moreira
Dec 4, 2012·International Journal of Molecular Sciences·Hanna-Maija HiltunenBernhard Grimm
Mar 7, 2014·Journal of Industrial Microbiology & Biotechnology·Valentyna Y YatsyshynAndriy A Sibirny
Jan 11, 2014·Molecular Plant-microbe Interactions : MPMI·Svetlana N YurgelWolfgang D Bauer
Oct 1, 2013·Oxidative Medicine and Cellular Longevity·Teresa Anna GiancasperoMaria Barile
Sep 1, 2012·Annual Review of Genetics·Norbert RollandDaphné Seigneurin-Berny
Aug 24, 2010·Trends in Plant Science·M Amparo Asensi-Fabado, Sergi Munné-Bosch
Jun 29, 2010·Journal of Plant Physiology·Min OuyangLixin Zhang
Jan 12, 2010·Mitochondrion·Enza Maria TorchettiMaria Barile
Mar 3, 2018·International Journal of Molecular Sciences·Yiyong ChenZiyin Yang
Oct 15, 2013·Chembiochem : a European Journal of Chemical Biology·Ilka HaaseMarkus Fischer
Dec 3, 2015·Acta Crystallographica. Section D, Biological Crystallography·Beatriz HerguedasMilagros Medina
Feb 28, 2018·Mycologia·Alexander GuhrAlfons R Weig
Aug 1, 2020·International Journal of Molecular Sciences·Patrick SchallBernhard Grimm
Dec 9, 2020·Journal of Integrative Plant Biology·Yufei LiJie Luo

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