Flavivirus sfRNA suppresses antiviral RNA interference in cultured cells and mosquitoes and directly interacts with the RNAi machinery

Virology
Stephanie L MoonJeffrey Wilusz

Abstract

Productive arbovirus infections require mechanisms to suppress or circumvent the cellular RNA interference (RNAi) pathway, a major antiviral response in mosquitoes. In this study, we demonstrate that two flaviviruses, Dengue virus and Kunjin virus, significantly repress siRNA-mediated RNAi in infected human cells as well as during infection of the mosquito vector Culex quinquefasciatus. Arthropod-borne flaviviruses generate a small structured non-coding RNA from the viral 3' UTR referred to as sfRNA. Analysis of infections with a mutant Kunjin virus that is unable to generate appreciable amounts of the major sfRNA species indicated that RNAi suppression was associated with the generation of the non-coding sfRNA. Co-immunoprecipitation of sfRNA with RNAi mediators Dicer and Ago2 suggest a model for RNAi suppression. Collectively, these data help to establish a clear role for sfRNA in RNAi suppression and adds to the emerging impact of viral long non-coding RNAs in modulating aspects of anti-viral immune processes.

References

Nov 16, 2004·Journal of Virology·Shihua Lu, Bryan R Cullen
Oct 18, 2005·Nature Structural & Molecular Biology·Jeffrey A ChaoJames R Williamson
Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kevin M MylesZach N Adelman
Apr 20, 2010·Nature Structural & Molecular Biology·Arabinda NayakRaul Andino
Aug 20, 2010·Journal of Virology·Anneke FunkAlexander A Khromykh
Aug 27, 2010·Journal of Virology·Patrícia A G C SilvaPeter J Bredenbeek
Mar 3, 2012·Wiley Interdisciplinary Reviews. RNA·Christopher Iain JonesSarah Faith Newbury
Aug 24, 2012·PLoS Pathogens·Joël T van MierloRonald P van Rij
Jul 24, 2013·Journal of Computer-aided Molecular Design·Mahmoud Kandeel, Yukio Kitade
Oct 15, 2013·Journal of Molecular Biology·Megan B KingsolverRichard W Hardy
Oct 17, 2013·Nature Reviews. Microbiology·Nathan Pumplin, Olivier Voinnet
Oct 22, 2013·Cell Host & Microbe·Bryan R CullenBenjamin R tenOever
Jan 16, 2014·Clinical and Experimental Vaccine Research·Yun Young GoChong-Kyo Lee
Mar 8, 2014·Biological Chemistry·Anne Dueck, Gunter Meister
Apr 20, 2014·Science·Erich G ChapmanJeffrey S Kieft
Jun 19, 2014·Nucleic Acids Research·Koen W R van CleefRonald P van Rij
Jul 18, 2014·PLoS Pathogens·Joël T van MierloRonald P van Rij
Oct 3, 2014·Nucleic Acids Research·Alfred W BronkhorstRonald P van Rij
Jan 8, 2015·Nature Structural & Molecular Biology·Jonathan J Ipsaro, Leemor Joshua-Tor
Jan 30, 2015·Wiley Interdisciplinary Reviews. RNA·Alex HeyamMichael Plevin

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Citations

Apr 6, 2016·Wiley Interdisciplinary Reviews. RNA·Mei-Ling LiGary Brewer
May 29, 2016·Virology Journal·Lorena BaviaClaudia Nunes Duarte Dos Santos
Oct 6, 2016·PLoS Neglected Tropical Diseases·Claire L DonaldAlain Kohl
Dec 30, 2016·PLoS Pathogens·Pascal MiesenRonald P van Rij
Jan 11, 2017·Biochemistry·Richard H G BaxterKathryn Krueger
Mar 9, 2017·Cellular Microbiology·Stephanie L CumberworthClaire L Donald
Mar 1, 2017·The Journal of Microbiology·Sang-Im Yun, Young-Min Lee
Dec 19, 2017·DNA and Cell Biology·Shih-Chia Yeh, Julien Pompon
Jun 5, 2018·PLoS Neglected Tropical Diseases·Flávia Viana FerreiraJoão Trindade Marques
May 12, 2018·Future Virology·Liang GuoPaul R Bohjanen
Aug 16, 2018·Wiley Interdisciplinary Reviews. RNA·Benjamin P Towler, Sarah F Newbury
Mar 14, 2019·Expert Reviews in Molecular Medicine·Pa WuGong Cheng
Sep 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Giel P GöertzGorben P Pijlman
May 1, 2019·Annual Review of Virology·Johanna B WithersJoan A Steitz
Dec 3, 2016·Biochemical Society Transactions·Amy L PashlerSarah F Newbury
Nov 10, 2017·The Journal of Biological Chemistry·Phillida A CharleyJeffrey Wilusz
Jun 4, 2020·Vaccines·Marie-France Martin, Sébastien Nisole
May 31, 2017·Frontiers in Microbiology·Patrice BruscellaMichele Trabucchi
May 6, 2017·The Biochemical Journal·Min-Sun Song, John J Rossi
Apr 19, 2018·Frontiers in Cellular and Infection Microbiology·Laura R H Ahlers, Alan G Goodman
Dec 20, 2018·Frontiers in Genetics·Clément MazeaudLaurent Chatel-Chaix
Feb 20, 2019·Viruses·Francine AzouzMukesh Kumar
Apr 25, 2019·Reviews in Medical Virology·Shaoqiong LiuWeifeng Shi
Apr 10, 2019·AMB Express·Cedric Chih Shen TanPaola Florez de Sessions
Nov 23, 2016·Frontiers in Microbiology·Michelle L PleetFatah Kashanchi
Jan 11, 2018·Nature Communications·Andrea MacFaddenJeffrey S Kieft
Feb 8, 2019·Frontiers in Microbiology·Yan-Jang S HuangDana L Vanlandingham
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Anna-Lena SteckelbergJeffrey S Kieft
Jun 20, 2019·ASN Neuro·Fernanda L CastroRenato S Aguiar
Nov 7, 2019·Viral Immunology·Branden R NelsonKristina M Adams Waldorf
Apr 6, 2019·RNA Biology·Ivar W DilwegRené C Olsthoorn
Oct 11, 2017·Frontiers in Immunology·Ruth E DruryAndrew J Pollard
Apr 30, 2019·Wiley Interdisciplinary Reviews. RNA·Shaun T CrossJeffrey Wilusz

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