Flavohemoglobin expression and function in Saccharomyces cerevisiae. No relationship with respiration and complex response to oxidative stress.

The Journal of Biological Chemistry
N Buisson, R Labbe-Bois

Abstract

The yeast Saccharomyces cerevisiae contains a flavohemoglobin, encoded by the gene YHB1, whose function is unclear. Previous reports presented evidence that its maximal expression requires disruption of mitochondrial respiration and that it plays a role in the response to oxidative stress. We have studied the expression of YHB1 in respiratory deficient cells and in cells exposed to various compounds causing oxidative stress. Several different strains and approaches (spectroscopic detection of the oxygenated form of Yhb1p, beta-galactosidase activity of a YHB1-lacZ fusion, and Northern blot analysis) were used to demonstrate that YHB1 expression and Yhb1p production are not increased by respiration deficiency. YHB1 expression was unchanged in cells challenged with antimycin A or menadione, while it decreased in cells exposed to H2O2, diamide, dithiothreitol, and Cu2+. Transcription of YHB1 is not under the control of the transcriptional factor Yap1p. These results do not support a participation of YHB1 in the genetic response to oxidative stress. We also analyzed the growth phenotypes associated with altered Yhb1p production using YHB1-deleted strains and strains that greatly overproduced Yhb1p. Yhb1p appears to protect cells ag...Continue Reading

References

Nov 14, 1977·Molecular & General Genetics : MGG·R Labbe-BoisT Bilinski
Oct 5, 1992·Journal of Molecular Biology·H IwaasaK Shikama
Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Mar 1, 1992·Microbiological Reviews·R S Zitomer, C V Lowry
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H Zhu, A F Riggs
May 6, 1991·Biochimica Et Biophysica Acta·A R Cross, O T Jones
May 25, 1990·Nucleic Acids Research·M E SchmittB L Trumpower
Nov 15, 1973·European Journal of Biochemistry·R OshinoB Hagihara
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·L Guarente, M Ptashne
Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D E Goldberg
Mar 24, 1995·The Journal of Biological Chemistry·M J CrawfordD E Goldberg
Mar 15, 1994·The Biochemical Journal·R M MouldT Brittain
Apr 26, 1996·Journal of Molecular Biology·M RizziM Bolognesi
May 21, 1996·Biochemistry·C BalagopalakrishnaJ M Rifkind
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·R C Hardison
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·C R AndersonW J Peacock
Jul 1, 1996·Journal of Bacteriology·M LaCelleM M Nakano
Feb 1, 1996·Molecular Microbiology·P Moradas-FerreiraW Mager
Jan 24, 1997·The Journal of Biological Chemistry·J A DeGrayR P Mason

❮ Previous
Next ❯

Citations

Sep 22, 2009·Canadian Journal of Microbiology·Taija S Koskenkorva-Frank, Pauli T Kallio
Apr 8, 2006·BMC Evolutionary Biology·Serge N VinogradovJacques R Vanfleteren
Sep 4, 2008·BMC Evolutionary Biology·Xavier BaillySerge N Vinogradov
May 3, 2000·Cell Structure and Function·M IijimaH Urushihara
Jul 24, 2008·Redox Report : Communications in Free Radical Research·Oleh V Lushchak, Volodymyr I Lushchak
Jul 24, 2008·Redox Report : Communications in Free Radical Research·Anna LewinskaGrzegorz Bartosz
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·L LiuJ S Stamler
Feb 18, 2016·International Journal of Proteomics·Chiranjit PanjaSanjay Ghosh
Mar 10, 2010·Biochemical and Biophysical Research Communications·Shengmin ZhouHirofumi Shoun
Nov 30, 2006·Redox Report : Communications in Free Radical Research·Anna Lewinska, Grzegorz Bartosz
Aug 23, 2002·The Journal of Biological Chemistry·Gen KobayashiKeiji Shikama
Sep 19, 2015·The Journal of Biological Chemistry·Paul R GardnerAlexander P Gardner
Sep 21, 2005·Journal of Biochemical and Molecular Toxicology·Polona Jamnik, Peter Raspor
Jun 25, 2002·The Journal of Biological Chemistry·Kyung-Won ParkDale A Webster
Feb 13, 1999·The Journal of Biological Chemistry·G SartoriG Carignani
Jun 18, 2005·Human Molecular Genetics·Pilar González-CaboFrancesc Palau
Sep 12, 2020·Trends in Biotechnology·Xinrui ZhaoJian Chen
May 26, 2004·Archives of Biochemistry and Biophysics·Michael L PendrakDavid D Roberts

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.