Flavone synthase II (CYP93B16) from soybean (Glycine max L.)

Phytochemistry
Judith FliegmannAxel Mithöfer

Abstract

Flavonoids are a very diverse group of plant secondary metabolites with a wide array of activities in plants, as well as in nutrition and health. All flavonoids are derived from a limited number of flavanone intermediates, which serve as substrates for a variety of enzyme activities, enabling the generation of diversity in flavonoid structures. Flavonoids can be characteristic metabolites, like isoflavonoids for legumes. Others, like flavones, occur in nearly all plants. Interestingly, there exist two fundamentally different enzymatic systems able to directly generate flavones from flavanones, flavone synthase (FNS) I and II. We describe an inducible flavone synthase activity from soybean (Glycine max) cell cultures, generating 7,4'-dihydroxyflavone (DHF), which we classified as FNS II. The corresponding full-length cDNA (CYP93B16) was isolated using known FNS II sequences from other plants. Functional expression in yeast allowed the detailed biochemical characterization of the catalytic activity of FNS II. A direct conversion of flavanones such as liquiritigenin, naringenin, and eriodictyol into the corresponding flavones DHF, apigenin and luteolin, respectively, was demonstrated. The enzymatic reaction of FNSII was stereosele...Continue Reading

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Citations

Nov 28, 2012·The Journal of Biological Chemistry·Anna Berim, David R Gang
Jan 9, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Björn Hamberger, Søren Bak
Jul 24, 2012·The Plant Cell·Kengo MorohashiErich Grotewold
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Jun 25, 2016·Plants·Nan JiangErich Grotewold
Sep 28, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Ramona TurcasJózsef Kaizer
Jul 27, 2018·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Haruna NagayoshiTsutomu Shimada
Jan 10, 2018·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Kensaku KakimotoTsutomu Shimada
Apr 2, 2019·Frontiers in Plant Science·Jessica D HohensteinGustavo C MacIntosh
Oct 14, 2020·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Tsutomu ShimadaF Peter Guengerich
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dóra Lakk-BogáthJózsef Kaizer
Jul 6, 2021·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Haruna NagayoshiTsutomu Shimada

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