Flavonoid fraction of guava leaf extract attenuates lipopolysaccharide-induced inflammatory response via blocking of NF-κB signalling pathway in Labeo rohita macrophages

Fish & Shellfish Immunology
Shib Sankar SenSe Chang Park

Abstract

Psidium guajava L. is a well-known traditional medicinal plant widely used in folk medicine. To explore the anti-inflammatory activity of the flavonoid fraction of guava leaf extract (FGLE), we investigated its ability to suppress the levels of inflammatory mediators elevated by lipopolysaccharide (LPS) in Labeo rohita head-kidney (HK) macrophages. HK macrophages of L. rohita were treated with LPS in the presence or absence of the FGLE. We examined the inhibitory effect of FGLE on LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production. The inhibitory effect of FGLE on nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were investigated by RT-PCR and western blot. The effect of FGLE on proinflammatory cytokines tumour necrosis factor alpha (TNF-α) or interleukin-1β (IL-1β) was also investigated by ELISA and RT-PCR. The phosphorylation of three mitogen activated protein kinases (MAPK) molecules ERK, JNK and p38 was analysed by western blot analysis. FGLE inhibited LPS-induced NO and PGE2 production. It also effectively inhibited TNF-α, IL-1β, IL-10, iNOS, and COX-2 production in a concentration-dependent manner. In addition, FGLE suppressed the mRNA expression levels of TNF-α and IL-1β in LPS-stimulated HK ma...Continue Reading

References

Feb 29, 2000·The Journal of Biological Chemistry·D J WadleighH R Herschman
Jan 31, 2002·Annual Review of Medicine·Marco E Turini, Raymond N DuBois
May 16, 2002·International Immunopharmacology·Bianca Rocca, Garret A FitzGerald
Oct 2, 2002·Bioscience, Biotechnology, and Biochemistry·Hidetoshi Arima, Gen-ichi Danno
Dec 23, 2003·Molecular Immunology·Cyril JaultJohanna Chluba
Jun 24, 2004·Journal of Cellular Biochemistry·N R MonksA B Pardee
Sep 27, 2005·The Journal of Pharmacology and Experimental Therapeutics·Jeong-Hyung LeeJung Joon Lee
Nov 22, 2005·FEBS Letters·Dimitar B IlievFrederick W Goetz
Jun 1, 2006·Cancer Science·Akihiko Yoshimura
Mar 6, 2007·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·B VerstakA Mansell
Mar 21, 2008·Journal of Ethnopharmacology·Rosa Martha Pérez GutiérrezRosario Vargas Solis
Feb 26, 2010·Nutrition & Metabolism·Yoriko Deguchi, Kouji Miyazaki
May 4, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuejuan ZhengXuetao Cao
Jun 30, 2010·BMC Complementary and Alternative Medicine·Tannaz BirdiNoshir Antia
Oct 12, 2010·Pharmacognosy Magazine·A M MetwallyS M El Sohafy
Jul 23, 2013·Fish & Shellfish Immunology·Dimitar B IlievJorunn B Jørgensen
Apr 5, 2014·Microbiology and Immunology·Halkare Suryanarayana VasaviPunchapady-Devasya Rekha
May 29, 2014·Infection and Immunity·Javier SantanderRoy Curtiss
Jul 6, 2014·Environmental Science and Pollution Research International·S JollyS Betoulle
Jul 11, 2014·Fish & Shellfish Immunology·Xiao-Li YinZhi-Xun Guo

❮ Previous
Next ❯

Citations

Mar 10, 2017·Redox Report : Communications in Free Radical Research·Noori Al-WailiKhelod Salom
Apr 27, 2017·International Journal of Molecular Sciences·Elixabet Díaz-de-CerioAntonio Segura-Carretero
Aug 29, 2018·Journal of Medical Virology·Hany KhalilSamir El-Masry
Oct 11, 2017·Pharmacognosy Reviews·Poonam G DaswaniTannaz J Birdi
May 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Adchara PrommabanSomdet Srichairatanakool

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Cardiovascular Inflammation

Inflammation plays a significant role in the development of cardiovascular diseases, an understanding of these endogenous processes is critical for evaluating the risks and potential treatment strategies. Discover the latest research on cardiovascular inflammation here.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.