Flavonoid synthesis in Petunia hybrida: partial characterization of dihydroflavonol-4-reductase genes

Plant Molecular Biology
M BeldA G Gerats

Abstract

In this paper we describe the organization and expression of the genes encoding the flavonoid-biosynthetic enzyme dihydroflavonol-4-reductase (DFR) in Petunia hybrida. A nearly full-size DFR cDNA clone (1.5 kb), isolated from a corolla-specific cDNA library was compared at the nucleotide level with the pallida gene from Antirrhinum majus and at the amino acid level with enzymes encoded by the pallida gene and the A1 gene from Zea mays. The P. hybrida and A. majus DFR genes transcribed in flowers contain 5 introns, at identical positions; the three introns of the A1 gene from Z. mays coincide with the first three introns of the other two species. P. hybrida line V30 harbours three DFR genes (A, B, C) which were mapped by RFLP analysis on three different chromosomes (IV, II and VI respectively). Steady-state levels of DFR mRNA in the line V30 follow the same pattern during development as chalcone synthase (CHS) and chalcone flavanone isomerase (CHI) mRNA. Six mutants that accumulate dihydroflavonols in mature flowers were subjected to Northern blot analysis for the presence of DFR mRNA. Five of these mutants lack detectable levels of DFR mRNA. Four of these five also show drastically reduced levels of activity for the enzyme UDPG...Continue Reading

References

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Citations

Feb 25, 2006·BioFactors·E D'Amico, G Perrotta
Jul 20, 2007·Plant Cell Reports·Takashi NakatsukaMasahiro Nishihara
Mar 27, 2010·Journal of Plant Research·Kazuko YoshidaMasaaki Sakuta
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Jul 21, 2011·Journal of Nucleic Acids·Lilian Cristina Baldon Aizza, Marcelo Carnier Dornelas

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