Flavonoids exert distinct modulatory actions on cyclooxygenase 2 and NF-kappaB in an intestinal epithelial cell line (IEC18).

British Journal of Pharmacology
R López-PosadasF Sánchez de Medina

Abstract

Cyclooxygenase 2 (COX-2) is involved in inflammatory bowel disease, but the effect of flavonoids at the intestinal epithelial level is unknown. We aimed to characterize the effect and structure-activity relationship of nine selected flavonoids on COX-2 expression in intestinal epithelial cell (IEC)18 cells. We also investigated the signal transduction pathway(s) responsible for the effects observed. Intestinal epithelial cell 18, a non-tumour cell line with intestinal epithelial phenotype, was used. COX-2 was measured by Western blot and the involvement of the NF-kappaB pathway assessed by Western blot, pharmacological inhibition, luciferase reporter assays and nuclear translocation experiments. The effect of flavonoids on COX-2 expression depended on the experimental conditions tested [non-stimulated and lipopolysaccharide (LPS)-stimulated]. Flavonoids caused an increase in COX-2 expression and NF-kappaB-dependent gene transcription under basal conditions. Conversely, under LPS stimulation flavonoids increased, decreased or did not affect COX-2 levels depending on the specific type. Variable effects were observed on extracellular signal regulated kinase/p38/c-Jun N-terminal kinase phosphorylation and p50/65 nuclear translocati...Continue Reading

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Citations

Jan 26, 2016·Journal of Dairy Science·J R KanwarG W Krissansen
Apr 29, 2015·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Wenping WangXinpu Yu
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May 26, 2018·Nutrition Reviews·Anthony F Juritsch, Régis Moreau
Mar 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lauryna PudziuvelyteJurga Bernatoniene

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