Flavonoids induce cell death in Leishmania amazonensis: in vitro characterization by flow cytometry and Raman spectroscopy

The Analyst
Morgana V AraújoMagna S Alexandre-Moreira

Abstract

Leishmaniasis comprises a group of infectious diseases with worldwide distribution, of which both the visceral and cutaneous forms are caused by Leishmania parasites. In the absence of vaccines, efficacious chemotherapy remains the basis for leishmaniasis control. The available drugs are expensive and associated with several secondary adverse effects. Due to these limitations, the development of new antileishmanial compounds is imperative, and plants offer various perspectives in this regard. The present study evaluated the in vitro leishmanicidal activity of flavonoids isolated from Solanum paludosum Moric. and investigated the mechanisms of cell death induced by them. These compounds were evaluated in vitro for their antileishmanial activity against Leishmania amazonensis promastigotes and they showed prominent leishmanicidal activity. The EtOAc fraction, gossypetin 3,7,8,4'-tetra-O-methyl ether (1), and kaempferol 3,7-di-O-methyl ether (3) were selected to be used in an in vitro assay against L. amazonensis amastigotes and cell death assays. The flavonoids (1) and (3) presented significant activity against L. amazonensis amastigotes, exhibiting the IC50 values of 23.3 ± 4.5 μM, 34.0 ± 9.6 μM, and 10.5 ± 2.5 μM for the EtOAc ...Continue Reading

References

Feb 5, 1991·Biochemistry·P F Devaux
Feb 13, 1999·The Journal of Biological Chemistry·X M SunG M Cohen
Oct 12, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·K DimasT Mavromoustakos
Feb 1, 2002·Natural Product Reports·M J Chan-Bacab, L M Peña-Rodríguez
Mar 5, 2003·Molecular and Biochemical Parasitology·Ananya BeraTushar Vaidya
Mar 8, 2003·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Sahar R GedaraAhmed F Halim
Apr 18, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·A C P OliveiraM M Coelho
Jul 10, 2003·Kinetoplastid Biology and Disease·Alain Debrabant, Hira Nakhasi
Sep 19, 2003·Phytotherapy Research : PTR·G VieiraE A Costa
Apr 8, 2004·Developmental Cell·Beth Levine, Daniel J Klionsky
Apr 21, 2004·European Journal of Biochemistry·Cinthya Assunção Guimarães, Rafael Linden
Nov 4, 2004·Biosensors & Bioelectronics·Ioan NotingherLarry L Hench
Jun 28, 2005·Current Opinion in Cell Biology·Fulvio Reggiori, Daniel J Klionsky
Oct 22, 2005·Biophysical Journal·James W ChanThomas Huser
Dec 29, 2006·Chemistry & Biodiversity·Paul A NguewaJosé M Pérez
Dec 30, 2006·Antimicrobial Agents and Chemotherapy·Iris L K WongLarry M C Chow
Aug 22, 2007·PloS One·Patrick WilliamsonRobert A Schlegel
Apr 2, 2010·Journal of Natural Products·Guy LewinThierry Cresteil
May 13, 2010·Journal of Biomedical Optics·Gobind DasEnzo Di Fabrizio
Mar 16, 2011·Journal of Agricultural and Food Chemistry·Sinead Eileen MilnerAnita Rose Maguire
Sep 6, 2011·European Journal of Medicinal Chemistry·Bhuwan B Mishra, Vinod K Tiwari
Oct 29, 2011·Molecular and Biochemical Parasitology·Rafael Balaña-FouceRosa M Reguera
Jun 14, 2012·PloS One·Jorge AlvarUNKNOWN WHO Leishmaniasis Control Team
Oct 3, 2012·Parasitology·Camila M AdadeThaïs Souto-Padrón
Apr 12, 2013·Chemistry & Biodiversity·Mariza Abreu MirandaJairo Kenupp Bastos
Sep 28, 2013·Essays in Biochemistry·Tohru Yonekawa, Andrew Thorburn
Oct 29, 2013·Journal of Medical Microbiology·Mohammad IslamuddinFarhat Afrin
Dec 1, 2012·International Journal for Parasitology. Drugs and Drug Resistance·Lucio H Freitas-JuniorJair L Siqueira-Neto
Apr 2, 2014·Antimicrobial Agents and Chemotherapy·Iris L K WongLarry M C Chow
Sep 27, 2014·Cell Death and Differentiation·Y Liu, B Levine

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Citations

Jul 1, 2020·Expert Opinion on Therapeutic Patents·Yasmim Maria Barbosa Gomes de CarvalhoLucindo José Quintans Júnior
Mar 26, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Bruna Taciane da Silva BortoletiWander Rogério Pavanelli

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Methods Mentioned

BETA
lavage
ELISA
flow cytometry
Assay
PCA

Software Mentioned

Muse® 1400 Analysis
[UNK] 1400
Waters MassLynx
MatLab
Graph Pad Prism
Muse® Cell Analyzer

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