PMID: 9528680Apr 7, 1998Paper

Flavonols--new fluorescent membrane probes for studying the interdigitation of lipid bilayers

Biochimica Et Biophysica Acta
O P BondarE S Rowe

Abstract

Two flavonols, 3-hydroxy-4'-dimethylaminoflavone (FME) and 3-hydroxy-4'-(15-azacrown-5) flavone (FRC) have been investigated as new fluorescence probes for studying the formation of the interdigitated gel phase in lipid bilayers. The formation of the interdigitated gel phase in the saturated symmetrical phosphatidylcholines (PCs) and phosphatidylethanol (Peth) in the presence of ethanol has been well studied. The present study examines the behavior of these new probes in PC-ethanol and Peth-ethanol systems, as well as in PC-cholesterol and Peth-cholesterol vesicles. The present results demonstrate that both flavonols give distinctively different spectra in interdigitated lipid compared to non-interdigitated lipids, when examined in lipids in which the interdigitation behavior is known. This makes them useful for determinations of the structural state of unknown lipids, and for following the transitions between interdigitated and non-interdigitated phases. However, in the presence of cholesterol, only FCR gave appropriate indications of interdigitation. The results with FME in the presence of cholesterol were not consistent with the known behavior of the lipids examined; instead, FME appears to be located preferentially in the c...Continue Reading

References

Jun 6, 1989·Biochimica Et Biophysica Acta·J M BoggsA Watts
Jan 1, 1988·Progress in Lipid Research·J L Slater, C H Huang
Aug 4, 1988·Biochimica Et Biophysica Acta·J A VeiroE S Rowe
Jun 16, 1987·Biochemistry·J MattaiG G Shipley
Dec 11, 1987·Biochimica Et Biophysica Acta·D A WilkinsonT J McIntosh
Aug 28, 1984·Biochemistry·T J McIntoshN A Porter
Oct 15, 1983·Journal of Molecular Biology·E N SerrallachG G Shipley
Sep 1, 1996·Biophysical Journal·O P Bondar, E S Rowe

❮ Previous
Next ❯

Citations

Jan 10, 2006·Journal of Fluorescence·Vasyl G PivovarenkoSergiy O Kosterin
Jul 16, 2005·Journal of Biochemical and Biophysical Methods·Zeynep TurkmenAlexander P Demchenko
Apr 28, 2004·Biophysical Journal·Andrey S KlymchenkoYves Mély
Sep 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Andrey S KlymchenkoAlexander P Demchenko
Mar 27, 2012·Phytochemistry·Yvonne Sichhart, Birgit Dräger
Jul 16, 2005·Analytical Biochemistry·Alexander P Demchenko
Jul 17, 2015·Journal of Fluorescence·Radhika KhannaRamesh C Kamboj
Jun 22, 2005·Trends in Biotechnology·Alexander P Demchenko
Nov 26, 2002·Chemistry & Biology·Andrey S KlymchenkoAlexander P Demchenko
Jan 23, 1999·Biophysical Journal·O P Bondar, E S Rowe
Oct 9, 2004·Biochimica Et Biophysica Acta·Andrey S KlymchenkoGuy Duportail
Nov 8, 2006·Journal of Molecular Recognition : JMR·Danièle AltschuhAlexander P Demchenko
Nov 2, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Dmytro A YushchenkoVasyl G Pivovarenko
Jan 4, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Gora M'BayeGuy Duportail
Nov 26, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Monalisa Mohapatra, Ashok Kumar Mishra
Apr 17, 2008·Molecular and Cellular Biochemistry·Yury S TarahovskyYuri A Kim
Mar 11, 2011·Physical Chemistry Chemical Physics : PCCP·Alberto MezzettiJean-Paul Cornard
Jun 16, 2006·The Journal of Physical Chemistry. a·Robson Valentim Pereira, Marcelo Henrique Gehlen
Aug 30, 2008·The Journal of Physical Chemistry. B·Ranjan DasYves Mély
Aug 26, 2006·The Journal of Organic Chemistry·Kristian DahlénKristina Luthman
Jul 13, 2006·The Journal of Physical Chemistry. a·Robson Valentim PereiraMarcelo Henrique Gehlen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.