Flavored little cigar smoke induces cytotoxicity and apoptosis in airway epithelia.

Cell Death Discovery
Arunava GhoshRobert Tarran

Abstract

Addition of flavors reduces the harsh taste of tobacco, facilitating the initiation and maintenance of addiction among youths. Flavored cigarettes (except menthol) are now banned. However, the legislation on little cigars remains unclear and flavored little cigars are currently available for purchase. Since inhaled tobacco smoke directly exerts toxic effects on the lungs, we tested whether non-flavored and flavored little cigar smoke exposure had the potential for harm in cultured pulmonary epithelia. We cultured Calu-3 lung epithelia on both 96-well plates and at the air-liquid interface and exposed them to smoke from non-flavored Swisher Sweets and flavored (sweet cherry, grape, menthol, peach and strawberry) Swisher Sweets little cigars. Irrespective of flavor, acute little cigar smoke exposure (10×35 ml puffs) significantly increased cell death and decreased the percentage of live cells. Chronic exposure (10×35 ml puffs per day for 4 days) of smoke to Calu-3 cultures significantly increased lactate dehydrogenase release, further indicating toxicity. To determine whether this exposure was associated with increased cell death/apoptosis, a protein array was used. Chronic exposure to smoke from all types of little cigars induce...Continue Reading

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Citations

May 23, 2019·Respiratory Research·Patrick J MooreRobert Tarran
Sep 3, 2020·American Journal of Respiratory Cell and Molecular Biology·Sabri H AbdelwahabMehmet Kesimer
Oct 7, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lynn CrosbyHans Rosenfeldt

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Methods Mentioned

BETA
protein array
Assay

Software Mentioned

STRING

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