FlbC is a putative nuclear C2H2 transcription factor regulating development in Aspergillus nidulans

Molecular Microbiology
Nak-Jung KwonJae-Hyuk Yu

Abstract

Asexual development (conidiation) in Aspergillus is governed by multiple regulators. Here, we characterize the upstream developmental activator FlbC in Aspergillus nidulans. flbC mRNA is detectable throughout the life cycle, at relatively high levels during vegetative growth, early asexual and late sexual developmental phases. The deletion of flbC causes a delay/reduction in conidiation, brlA and vosA expression, and conidial germination. While overexpression of flbC (OEflbC) does not elaborate conidiophores, it inhibits hyphal growth and activates expression of brlA, abaA and vosA, but not wetA. FlbC is conserved in filamentous Ascomycetes containing two C(2) H(2) zinc fingers at the C-terminus and a putative activation domain at the N-terminus. FlbC localizes in the nuclei of both hyphae and developmental cells. Localization and expression of FlbC are not affected by the absence of FlbB or FlbE, and vice versa. Importantly, overexpression of flbC causes growth inhibition and activation of abaA and vosA in the absence of brlA and abaA respectively. In vitro DNA-binding assay reveals that FlbC binds to the brlA, abaA and vosA, but not the wetA, promoters. In summary, FlbC is a putative nuclear transcription factor necessary for...Continue Reading

References

Nov 1, 1991·Molecular and Cellular Biology·C E Dowzer, J M Kelly
Jan 1, 1991·Molecular and Cellular Biology·M A Marshall, W E Timberlake
Jan 1, 1991·Methods in Enzymology·F Sherman
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T H Adams, W E Timberlake
Dec 1, 1971·Journal of General Microbiology·S D Martinelli, A J Clutterbuck
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Apr 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·T H AdamsJ H Yu
Apr 1, 1999·Biotechnology and Bioengineering·T ChristiansenJ Nielsen
Sep 12, 2002·Fungal Genetics and Biology : FG & B·Hee KimKeon Chae
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Monika PapworthAaron Klug
Jan 1, 1953·Advances in Genetics·G PONTECORVOA W J BUFTON
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Jae-Hyuk YuClaudio Scazzocchio
Feb 8, 2005·Journal of Clinical Microbiology·James McBrideCraig W Roberts
Oct 13, 2006·Eukaryotic Cell·Jae-Hyuk YuJeong-Ah Seo
Oct 13, 2006·Eukaryotic Cell·Jae-Hyung Mah, Jae-Hyuk Yu
Apr 5, 2007·Nature Protocols·Edyta SzewczykBerl Oakley

❮ Previous
Next ❯

Citations

Jun 1, 2011·Genetics·Carmen Ruger-HerrerosDavid Canovas
Jan 20, 2016·Current Genetics·Elixabet Oiartzabal-AranoOier Etxebeste
Nov 3, 2010·Trends in Microbiology·Oier EtxebesteUnai Ugalde
Sep 8, 2010·Fungal Genetics and Biology : FG & B·Nak-Jung KwonJae-Hyuk Yu
Oct 25, 2012·Current Opinion in Microbiology·Hee-Soo Park, Jae-Hyuk Yu
Dec 23, 2014·Research in Microbiology·Mingshuang WangHongye Li
Nov 19, 2011·FEMS Microbiology Reviews·Paul S Dyer, Céline M O'Gorman
Jun 28, 2015·BMC Genomics·Tamás EmriIstván Pócsi
Mar 2, 2013·Studies in Mycology·P KrijgsheldH A B Wösten
Sep 21, 2016·Fungal Biology·Perng-Kuang ChangJosé Diana Di Mavungu
Jun 6, 2018·Microorganisms·Vera Meyer, Sascha Jung
Jul 2, 2016·Scientific Reports·Mi-Kyung LeeJae-Hyuk Yu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.